Transmembrane protein 18 enhances the tropism of neural stem cells for glioma cells
| Autor: | Yuan Hong Yu, Ying Zhao, Jerome Boulaire, Doreen Siu Yi Leung, Sohail Ahmed, Shu Wang, Jaana Jurvansuu |
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| Rok vydání: | 2008 |
| Předmět: |
Cancer Research
Candidate gene Blotting Western Biology Tropism Cell Line Mice Cell Movement Glioma medicine Animals Humans Neoplasm Invasiveness Gene Silencing RNA Messenger Gene Gene Library Brain Neoplasms Reverse Transcriptase Polymerase Chain Reaction Membrane Proteins Cell migration Fibroblasts medicine.disease Molecular biology Transmembrane protein In vitro Neural stem cell Peptide Fragments nervous system diseases Rats Mice Inbred C57BL Oncology Culture Media Conditioned Cancer research NIH 3T3 Cells Neoplastic Stem Cells Female Rabbits |
| Zdroj: | Cancer research. 68(12) |
| ISSN: | 1538-7445 |
| Popis: | The failure of current glioma therapies is mainly due to the ability of the tumor cells to invade extensively the surrounding healthy brain tissue, hence escaping localized treatments. Neural stem cells (NSC) are able to home in on tumor foci at sites distant from the main tumor mass, possibly enabling treatment of scattered glioma clusters. To make the strategy more effective, we performed a cDNA expression library screening to identify the candidate genes that once overexpressed would enhance the tropism of NSCs for gliomas. Here, we show that a previously unannotated gene, the one encoding transmembrane protein 18 (TMEM18), is one such gene. Overexpression of TMEM18 was seen in the current study to provide NSCs and neural precursors an increased migration capacity toward glioblastoma cells in vitro and in the rat brain. Functional inactivation of the TMEM18 gene resulted in almost complete loss of the migration activity of these cells. Thus, TMEM18 is a novel cell migration modulator. Overexpression of this protein could be favorably used in NSC-based glioma therapy. [Cancer Res 2008;68(12):4614–22] |
| Databáze: | OpenAIRE |
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