Male subfertility effects of sub-chronic ethanol exposure during stress in a rat model
| Autor: | Mohammad Reza Jafarzadeh Shirazi, Farhad Rahmanifar, Amin Tamadon, Reza Fozooni, Bahia Namavar Jahromi, Arezoo Khoradmehr, Saman Saedi, Zahra Khodabandeh, Morteza Anvari |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty Health (social science) Apoptosis Semen analysis Toxicology Biochemistry Rats Sprague-Dawley Behavioral Neuroscience chemistry.chemical_compound Internal medicine Testis medicine Animals Testosterone Spermatogenesis Infertility Male Kisspeptins Ethanol TUNEL assay medicine.diagnostic_test business.industry General Medicine Epididymis Sperm Rats Endocrinology medicine.anatomical_structure Neurology chemistry Sperm Motility Receptor Melanocortin Type 4 business Stress Psychological Hormone |
| Zdroj: | Alcohol. 96:63-71 |
| ISSN: | 0741-8329 |
| DOI: | 10.1016/j.alcohol.2021.08.003 |
| Popis: | Background Stressful conditions increase alcohol consumption in men. Clinical studies link disruption of the neuroendocrine stress system with alcoholism, but the effect of alcohol in a stress condition on male fertility is still relatively poorly understood. This project was undertaken to evaluate the effect of sub-chronic alcohol in a stress condition on male fertility in a rat model. Methods Male Sprague–Dawley rats were randomly divided into a control group, a stress group that was exposed to restraint stress, an ethanol group that was injected with ethanol daily, and a stress + ethanol group that was injected with ethanol daily and was exposed to restraint stress, simultaneously. Furthermore, testis tissue was evaluated histomorphometrically and immunohistochemically for apoptosis using a TUNEL assay after 12 days. Epididymis sperm analysis was done. Blood cortisol and testosterone were measured and expression of hypothalamic kisspeptin (Kiss1), RFRP-3, and MC4R mRNA were evaluated. Results Ethanol exposure during restraint stress did not alter body weight. Ethanol exposure decreased the cellular diameter and area, and stress increased the cellular diameter and area, in comparison with the control group. In the stress group, in comparison with the other groups, the number of seminiferous tubules decreased and the numerical density of seminiferous tubules increased. In addition, ethanol exposure and/or stress reduced semen analysis parameters (sperm viability and motility), but did not change serum testosterone concentrations. Apoptosis increased in spermatogonia with ethanol exposure, but spermatocytes were not affected. Our data present the novel finding that ethanol and stress reduced hypothalamic Kiss1 mRNA expression, while ethanol exposure decreased hypothalamic RFRP-3 and MC4R mRNA expression. Conclusions Ethanol decreased cortisol hormone level during the restraint stress condition and attenuated hypothalamic reproductive-related gene expressions. Therefore, ethanol exposure may induce reduction of sperm viability, increased sperm mortality, and increased apoptosis, with long-term effects, and may induce permanent male subfertility. |
| Databáze: | OpenAIRE |
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