Ultraviolet radiation stimulates the release of arachidonic acid from mammalian cells in culture
| Autor: | Vincent A. De Leo, Leonard C. Harber, Deborah Hanson, I. B. Weinstein |
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| Rok vydání: | 1985 |
| Předmět: |
Ultraviolet Rays
Phospholipid chemistry.chemical_element Stimulation Arachidonic Acids Calcium Biochemistry Cell Line chemistry.chemical_compound Membrane Lipids Mice Phospholipase A2 Animals Physical and Theoretical Chemistry Mice Inbred C3H Arachidonic Acid integumentary system biology General Medicine Metabolism chemistry Cell culture biology.protein Liberation Arachidonic acid |
| Zdroj: | Photochemistry and photobiology. 41(1) |
| ISSN: | 0031-8655 |
| Popis: | — C3H 10T½ cells in culture were prelabelled with [3H]arachidonic acid and exposed to UVB radiation. Almost immediately after irradiation cells released labelled arachidonate metabolites into media in a dose dependent manner. This release was inhibited by removing calcium ions from the system and by the addition of dexamethasone and parabromophenacyl bromide to the system. This suggests that the UVB stimulated release of arachidonic acid from membrane phospholipids is, in part, mediated by a phospholipase A2 enzyme system. Thin layer chromatographic examination of media extracts revealed a dose dependent UVB stimulation of prostaglandin production by cultured cells. |
| Databáze: | OpenAIRE |
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