Effect of metronidazole ophthalmic solution on corneal neovascularization in a rat model
| Autor: | Flor Diana Yokoay Claros-Chacaltana, José Luiz Laus, Marcela Aldrovani, Alexandre Augusto Franchi de Barros Sobrinho, Thais Guimarães Morato Abreu, Gisele Pereira Valdetaro, Karina Kamachi Kobashigawa, Ivan Ricardo Martinez Padua |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
medicine.medical_specialty Time Factors genetic structures medicine.medical_treatment Rat model Chemical burn Sham group Neovascularization Cornea 03 medical and health sciences 0302 clinical medicine Anti-Infective Agents Ophthalmology Metronidazole Burns Chemical medicine Animals Corneal Neovascularization Rats Wistar Saline Dose-Response Relationship Drug business.industry medicine.disease eye diseases Rats Disease Models Animal Eye Burns medicine.anatomical_structure Treatment Outcome Corneal neovascularization 030221 ophthalmology & optometry sense organs Angiogenesis medicine.symptom Ophthalmic Solutions business 030217 neurology & neurosurgery medicine.drug Follow-Up Studies |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2018-12-11T17:19:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-04-26 Purpose: To evaluate the effect of metronidazole ophthalmic solutions on corneal neovascularization (CNV) in a rat model. Methods: A chemical burn was created in the right central cornea of 40 rats. Animals were randomized and distributed into four study groups (n = 10 rats per group) designated Met_0.1%, Met_0.5%, sham, and untreated groups. Chemical-burned corneas in the Met_0.1% and Met_0.5% groups received ophthalmic solutions of 0.1 and 0.5% metronidazole, respectively. Corneas in the sham group received phosphate-buffered saline (metronidazole diluent). All treated eyes received ophthalmic solution at intervals of 6 h, for up to 30 days. Untreated corneas received no treatment. CNV was evaluated postinjury using corneal photographs at different evaluation time points. The main CNV outcome measures were: burn intensity, index of CNV, and percentage of vascularized corneal area. Five rats from each group were euthanized, on days 15 and 30; the samples were collected for histological analyses. Differences with P < 0.05 were considered significant. Results: CNV was observed in the eyes from day 7 postinjury. However, the indices of CNV for the Met_0.1% and Met_0.5% groups were smaller than those for the sham and untreated groups (P < 0.05). Furthermore, corneas treated with 0.1 or 0.5% metronidazole had smaller vascularized areas compared to control corneas. On histological study, the presence of blood vessels confirmed clinical outcomes. Conclusions: Regular instillation of 0.1 or 0.5% metronidazole had a significant inhibitory effect for CNV on chemical burns induced in a rat model. Ophthalmology Unit Department of Veterinary Clinics and Surgery School of Agricultural and Veterinarian Sciences (FCAV) São Paulo State University (UNESP), Via de acesso Prof. Paulo Donato Castellane s/n Ophthalmology Unit Department of Veterinary Clinics and Surgery School of Agricultural and Veterinarian Sciences (FCAV) São Paulo State University (UNESP), Via de acesso Prof. Paulo Donato Castellane s/n |
| Databáze: | OpenAIRE |
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