Prenatal and postnatal diagnoses of thalassemias and hemoglobinopathies by HPLC
| Autor: | Jew Chowthaworn, Busara Sae-Ngow, Wanna Muangsapaya, Rungrat Sriphanich, Raewadee Wisedpanichkij, Ahnond Bunyaratvej, Chris Dewaele, Suthat Fucharoen, Pranee Winichagoon, Sujin Kanokpongsakdi, Warangkana Oncoung, Anong Piankijagum |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Hemolytic anemia congenital hereditary and neonatal diseases and abnormalities Pediatrics medicine.medical_specialty Hemoglobins Abnormal Thalassemia Clinical Biochemistry Southeast asia alpha-Thalassemia Pregnancy Prenatal Diagnosis hemic and lymphatic diseases medicine Humans South east asia Hemoglobin A2 Medical diagnosis Chromatography High Pressure Liquid business.industry beta-Thalassemia Biochemistry (medical) Infant Newborn Fetal Blood Thailand medicine.disease Hemoglobinopathies Phenotype Hemoglobinopathy Cord blood Female Hemoglobin business |
| Zdroj: | Clinical Chemistry. 44:740-748 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/44.4.740 |
| Popis: | The conventional approach to qualitative and quantitative analyses of hemoglobin (Hb) molecules for the diagnoses of hemoglobinopathies requires a combination of tests. We used an automated HPLC (VARIANT™) system to study α-thalassemia and β-thalassemia syndromes in Thailand. The beta-thalassemia short program is applicable to the diagnosis of α-thalassemia and β-thalassemia disorders, including Hb H, EA Bart’s disease, and EF Bart’s disease, in adults, newborns, and fetuses. The system cannot quantify accurately certain Hb molecules, such as Hb H and Hb Bart’s. The alpha-thalassemia short program was therefore developed and used to quantify Hb Bart’s to detect α-thalassemia genotypes in cord blood. This automated HPLC system is an alternative approach to the diagnosis of complicated thalassemia syndromes in Thailand and Southeast Asia. |
| Databáze: | OpenAIRE |
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