A simple and sensitive electrochemiluminescence aptasensor for determination of ochratoxin A based on a nicking endonuclease-powered DNA walking machine
Autor: | Jin Chen, Songqin Liu, Wei Wei, Xiaolin Xu, Min Wei, Chunlei Wang, Ensheng Xu |
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Rok vydání: | 2019 |
Předmět: |
Ochratoxin A
Signal amplification technique Biosensing Techniques Sulfides 01 natural sciences Analytical Chemistry Endonuclease chemistry.chemical_compound 0404 agricultural biotechnology Semiconductor quantum dots Limit of Detection Quantum Dots Cadmium Compounds Electrochemiluminescence DNA Breaks Single-Stranded Electrodes Detection limit Chromatography biology Chemistry 010401 analytical chemistry technology industry and agriculture 04 agricultural and veterinary sciences General Medicine Aptamers Nucleotide Carbocyanines Endonucleases Ochratoxins 040401 food science 0104 chemical sciences Linear range Luminescent Measurements biology.protein DNA Food Science |
Zdroj: | Food Chemistry. 282:141-146 |
ISSN: | 0308-8146 |
Popis: | Ochratoxin A (OTA) poses a serious threat to the health of human beings and animals. In this paper, a simple and sensitive electrochemiluminescence (ECL) aptasensor was constructed to detect OTA based on electrochemiluminescence resonance energy transfer (ECL-RET) and a nicking endonuclease-powered DNA walking machine. Originally, the signal of cadmium sulfide semiconductor quantum dots (CdS QDs) was quenched efficiently by Cy5. After the addition of OTA, the walker autonomously hybridized with Cy5-labeled DNA and released plenty of Cy5-DNA from the electrode surface with the help of a nicking endonuclease. As a result, the signal of CdS QDs recovered efficiently. As an artificial and popular signal amplification technique, the DNA walking machine greatly improved the sensitivity. Under optimal conditions, the aptasensor not only detected OTA in a linear range from 0.05 nM to 5 nM with a detection limit of 0.012 nM (S/N = 3), but also showed an excellent selectivity for OTA over other mycotoxins. |
Databáze: | OpenAIRE |
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