Urine protein profile of IgA nephropathy patients may predict the response to ACE-inhibitor therapy
| Autor: | Diego Centonze, Salvatore Di Paolo, Carmen Palermo, Maria Teresa Rocchetti, Marta Centra, Grazia Bortone, Massimo Papale, Loreto Gesualdo, Elena Ranieri |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Spectrometry Mass Electrospray Ionization medicine.medical_specialty Proteome Urinary system Protein Array Analysis Renal function Angiotensin-Converting Enzyme Inhibitors urologic and male genital diseases Biochemistry Gastroenterology Nephropathy Predictive Value of Tests Tandem Mass Spectrometry Internal medicine medicine Humans Electrophoresis Gel Two-Dimensional Molecular Biology Chromatography High Pressure Liquid Kininogen Proteinuria biology Chemistry Glomerulonephritis IGA Angiotensin-converting enzyme Glomerulonephritis Middle Aged medicine.disease Endocrinology ACE inhibitor biology.protein Female medicine.symptom medicine.drug |
| Zdroj: | PROTEOMICS. 8:206-216 |
| ISSN: | 1615-9861 1615-9853 |
| DOI: | 10.1002/pmic.200700492 |
| Popis: | This study was aimed at the search of urinary biomarkers which might help to predict the clinical response of IgA nephropathy (IgAN) patients to angiotensin converting enzyme inhibitors (ACEi). First, we studied the urinary proteome of 18 IgAN patients (toward 20 healthy controls) who had been chronically treated with ACEi by using 2-D PAGE coupled to nano-HPLC-ESI-MS/MS analysis. We identified 3 proteins, kininogen (p = 0.02), inter-alpha-trypsin-inhibitor heavy chain 4 (35 kDa fragment) (p = 0.02) and transthyretin (p0.0001), whose urinary excretion was different in IgAN patients' responders when compared to those who had not responded to ACEi. A reduction of daily proteinuria50% and a stable renal function over time were used to classify patients as responders. Then, we adopted immunoblotting to confirm the predictive power of one of the above proteins, kininogen, in 20 patients with biopsy-proven IgAN, before starting any therapy. Thus, we confirmed that very low levels of kininogen urine excretion were indeed predictive of an inadequate or absent clinical response to ACEi therapy of IgAN patients, after 6-month follow-up. Concluding, the analysis of urine proteome of IgAN patients generated a set of proteins which distinguished subjects responsive to ACEi from those unresponsive to the inhibition of renin-angiotensin system (RAS). |
| Databáze: | OpenAIRE |
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