A statistical approach for optimization of alkaline lipase production by ascidian associated—Halobacillus trueperi RSK CAS9
| Autor: | Chinnathambi Stalin, Ramamoorthy Sathishkumar, Kathirvel Iyappan, Gnanakkan Ananthan |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
biology
Central composite design lcsh:Biotechnology Halobacillus trueperi Haloalkaline lipase biology.organism_classification Applied Microbiology and Biotechnology Catalysis Serine chemistry.chemical_compound Response surface methodology chemistry Biochemistry lcsh:TP248.13-248.65 Didemnum candidum biology.protein Food science Lipase PMSF Bacteria Marine wastes Biotechnology |
| Zdroj: | Biotechnology Reports, Vol 8, Iss C, Pp 64-71 (2015) |
| ISSN: | 2215-017X |
| DOI: | 10.1016/j.btre.2015.09.002 |
| Popis: | A marine ascidian-associated bacterium, Halobacillus trueperi RSK CAS9, was optimized for lipase production by response surface methodology using marine waste as substrate. The central composite design was employed, and the optimal medium constituents for maximum lipase production (1355.81 U/ml) were determined to be tuna powder (14.58 g/l), olive oil (5.05 ml/l); NaCl (72.42 g/l), temperature (45 °C) and pH 9.0. An alkaline lipase was purified to 8.46 fold with 1193.59 U mg −1 specific activities with the molecular weight of 44 kDa. The activity was substantially inhibited by EDTA and PMSF, indicating that it was a metalloenzyme serine residue which was essential for catalytic activity. Thus, lipase production by microbial conversion of marine fish wastes in this study suggested its potential utilization for the production of high value products. |
| Databáze: | OpenAIRE |
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