Alveolar subphase pH in the lungs of anesthetized rabbits
| Autor: | J A Clements, D W Nielson, J Goerke |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Multidisciplinary
Lung Chromatography Chemistry Alveolar Epithelium Analytical chemistry Anesthesia General Hydrogen-Ion Concentration respiratory system Lung surface Pulmonary Alveoli Microelectrode medicine.anatomical_structure Torr Electrode Methods Potentiometry medicine Animals Rabbits Microelectrodes Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 78:7119-7123 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.78.11.7119 |
| Popis: | We measured the pH of the alveolar subphase fluid by puncturing the most superficial alveoli of the exposed lungs of anesthetized rabbits with H+-selective and nonselective KCl microelectrodes. In these experiments, we bathed the lung surface with paraffin oil or buffered Ringer's solutions that had a CO2 tension of 40 Torr (1 Torr = 133.3 Pa) and found an alveolar pH of 6.92 +/- 0.01 (mean +/- sEM). When the pH of the surface buffer was below 6.7 or above 7.5, alveolar pH varied with surface buffer pH. With the nonselective electrode, we did not find a significant electrical potential difference between the alveolar fluid and the pleural surface. These results are consistent with active transport of H+ (or HCO3(-) across alveolar epithelium. |
| Databáze: | OpenAIRE |
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