Repeated exposure to low levels of sulfur dioxide (SO2) enhances the development of ovalbumin-induced asthmatic reactions in guinea pigs
| Autor: | Yoon Keun Kim, Sang Rok Lee, Sang Heon Cho, You Young Kim, Kyung Up Min, Jae Kyoung Park |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine Allergy Ovalbumin Guinea Pigs Immunology Physiology medicine.disease_cause Severity of Illness Index complex mixtures Guinea pig Leukocyte Count Prevalence medicine Animals Sulfur Dioxide Immunology and Allergy Sensitization Asthma Air Pollutants medicine.diagnostic_test biology business.industry Aeroallergen Environmental Exposure respiratory system Airway obstruction medicine.disease respiratory tract diseases Eosinophils Bronchoalveolar lavage medicine.anatomical_structure biology.protein business Bronchoalveolar Lavage Fluid |
| Zdroj: | Annals of Allergy, Asthma & Immunology. 86:62-67 |
| ISSN: | 1081-1206 |
| DOI: | 10.1016/s1081-1206(10)62358-7 |
| Popis: | Sulfur dioxide (SO2) is one of the major air pollutants. It is known to aggravate asthma symptoms in human beings, but few studies have focused on the effects of SO2 upon the development of bronchial asthma in animal models.This study was undertaken to evaluate the role of SO2 upon the development of ovalbumin (OA)-induced asthmatic reactions in guinea pigs.Guinea pigs were divided into four groups: (1) OA- and SO2-exposed group (n = 12), (2) SO2-exposed group (n = 12), (3) OA-exposed group (n = 11), and (4) saline-exposed group (n = 7). Guinea pigs of the first and second groups were exposed to 0.1 ppm SO2 for 5 hours a day on 5 consecutive days. Guinea pigs in the first and third groups inhaled 0.1% OA aerosols for 45 minutes a day on days 3, 4, and 5. One week after the sensitization procedure, all the guinea pigs underwent bronchial challenge with 1.0% OA aerosols, using unrestricted whole-body plethysmography. Bronchoalveolar lavage and histopathologic examination were performed 24 hours after the bronchial challenge.Increases in enhanced pause (Penh), as an index of airway obstruction, after the bronchial challenge was significantly higher in OA- and SO2-exposed group (group 1) than the other groups (P.05, respectively). Eosinophil counts in bronchoalveolar lavage fluids were also significantly higher in group 1 than in the other groups (P.05, respectively). Histopathologic findings of bronchial and lung tissue in the group 1 showed an infiltration of inflammatory cells, bronchiolar epithelial damage, and mucus and cell plug in the lumen, but no significant abnormalities were observed in the other groups.These results indicate that repeated exposure to low levels of sulfur dioxide may enhance the development of ovalbumin-induced asthmatic reactions in guinea pigs. |
| Databáze: | OpenAIRE |
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