Human glioblastoma cells produce granulocyte-macrophage colony-stimulating factor in vitro, but not in vivo, without expressing its receptor
| Autor: | Junichi Murata, Mitsuhiro Tada, Toshimitsu Aida, Yutaka Sawamura, Hiroshi Abe, Shiro Sakuma, Masako Sudo |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
medicine.diagnostic_test
business.industry medicine.medical_treatment Receptor expression Infant Newborn Granulocyte-Macrophage Colony-Stimulating Factor medicine.disease Molecular biology Flow cytometry Granulocyte macrophage colony-stimulating factor Cytokine Cell culture Receptors Granulocyte-Macrophage Colony-Stimulating Factor Glioma Immunology medicine Tumor Cells Cultured Humans Surgery Tumor necrosis factor alpha Neurology (clinical) Receptor business Glioblastoma medicine.drug |
| Zdroj: | Neurologia medico-chirurgica. 33(9) |
| ISSN: | 0470-8105 |
| Popis: | Granulocyte-macrophage colony-stimulating factor (GM-CSF) production and receptor expression by human glioblastomas was studied. Enzyme-linked immunosorbent assay showed four of 10 glioblastoma cell lines spontaneously released GM-CSF (2.9-9.2 pg GM-CSF protein/ml culture medium), which was enhanced by stimulation with tumor necrosis factor-alpha (TNF) (10 U/ml) up to 410 pg/ml. TNF also induced secretion of GM-CSF by another cell line. Northern blot analysis identified increasing GM-CSF gene expression by cells following TNF stimulation. However, no GM-CSF protein was detectable in the cerebrospinal fluid of three malignant glioma patients. Intratumoral administration of TNF in the patients also failed to stimulate GM-CSF levels in the cerebrospinal fluid. A binding assay using flow cytometry with biotinylated GM-CSF and Scatchard analysis using 125I-labeled GM-CSF failed to demonstrate GM-CSF receptor expression on the 13 cell lines. Exogenous GM-CSF stimulation had no effect on production of prostaglandin E2, interleukin-6, or interleukin-8 by glioma cells. Human glioblastoma cells secrete GM-CSF without expressing the receptor in vitro, but there was no evidence of GM-CSF production in vivo. |
| Databáze: | OpenAIRE |
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