An Immunohistochemical Study of an Autosomal Dominant Feline Rod/Cone Dysplasia (Rdy Cats)
| Autor: | Robert A. Alexander, Philip J. Luthert, Alan C. Bird, K. C. Barnett, Nhv Chong |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Retinal degeneration
Pathology medicine.medical_specialty Opsin Time Factors genetic structures Synaptophysin Outer plexiform layer Apoptosis Cell Count Biology Retina Cellular and Molecular Neuroscience Immunolabeling Retinal Rod Photoreceptor Cells Glial Fibrillary Acidic Protein In Situ Nick-End Labeling medicine Animals Outer nuclear layer Glial fibrillary acidic protein Retinal Degeneration Rod Opsins medicine.disease Immunohistochemistry Sensory Systems Disease Models Animal Ophthalmology medicine.anatomical_structure Animals Newborn Cats biology.protein sense organs Photoreceptor Cells Vertebrate |
| Zdroj: | Experimental Eye Research. 68:51-57 |
| ISSN: | 0014-4835 |
| DOI: | 10.1006/exer.1998.0580 |
| Popis: | An autosomal dominant, early onset feline model of rod/cone dysplasia has been described. The clinical features, light and electron microscopy, and the electrophysiology were documented. We have now examined in more detail the histopathological and immunohistochemical changes during the early phase of the disease using antibodies against opsin, synaptophysin, glial fibrillary acidic protein (GFAP) and an epithelial marker (MNF118). We have also demonstrated programmed cell death by a modified TUNEL (Terminal deoxynucleotidyl transferase, Uridine triphosphate, Nick End Labelling) technique. In the Rdy cats, there was significant photoreceptor degeneration between 5 and 17 weeks of age. The TUNEL-labeled cell and pyknotic cell counts in the outer nuclear layer peaked at around 9 weeks of age. Accumulation of opsin in the entire outer nuclear layer of the retina was noted with opsin-immunolabeled rod neurite sprouting. There was a reduction in synaptophysin immunoreactivity in the outer plexiform layer. The Muller cells were activated and expressed GFAP. No significant change of immunolabeling of MNF118 was found. These findings closely parallel those seen in human RP. |
| Databáze: | OpenAIRE |
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