α-L-fucosidase from bull seminal plasma: Its purification and acrosome reaction promoting property
| Autor: | K. Arbtan, G.H. Takei, Prakash N. Srivastava, T.T.F. Huang, Ryuzo Yanagimachi |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Male
Guinea Pigs Acrosome reaction Biophysics Biochemistry High-performance liquid chromatography Guinea pig Human fertilization Semen medicine Animals Molecular Biology Polyacrylamide gel electrophoresis alpha-L-Fucosidase chemistry.chemical_classification Chromatography Cell Biology Spermatozoa In vitro Enzyme medicine.anatomical_structure chemistry Fertilization Gamete Cattle Acrosome |
| Zdroj: | Biochemical and Biophysical Research Communications. 137:1061-1068 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(86)90333-5 |
| Popis: | α-L-fucosidase was purified from the bull seminal plasma by chromatography on DEAE-disk, octyl sepharose hydrophobic column and HPLC. The enzyme appeared to be pure as judged by the polyacrylamide gel electrophoresis both under the nondenaturing and denaturing conditions. The pure enzyme promoted the acrosome reaction of guinea pig spermatozoa in vitro. This is the first report showing that an acrosomal enzyme induces acrosome reaction which is an essential pre-requisite for the gamete interaction and fertilization. |
| Databáze: | OpenAIRE |
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