TCTP regulates spindle assembly during postovulatory aging and prevents deterioration in mouse oocyte quality
| Autor: | Xiang-Shun Cui, Jae Man Lee, Jeong Su Oh, Jing Guo, Jae-Sung Kim, Hyuk-Joon Jeon |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty media_common.quotation_subject Spindle Apparatus Biology Luteal Phase Oogenesis 03 medical and health sciences Mice Human fertilization Internal medicine medicine Biomarkers Tumor Animals Blastocyst Molecular Biology Ovulation Cells Cultured Cellular Senescence media_common Gene knockdown Embryogenesis Tumor Protein Translationally-Controlled 1 Cell Biology Oocyte Cell biology Spindle apparatus 030104 developmental biology Endocrinology medicine.anatomical_structure Oocytes Female |
| Zdroj: | Biochimica et biophysica acta. Molecular cell research. 1864(7) |
| ISSN: | 0167-4889 |
| Popis: | If no fertilization occurs for a prolonged time following ovulation, oocytes experience a time-dependent deterioration in quality both in vivo and in vitro due to processes called postovulatory aging. Because the postovulatory aging of oocytes has marked detrimental effects on embryo development and offspring, many efforts have been made to unveil the underlying mechanisms. Here we showed that translationally controlled tumor protein (TCTP) regulates spindle assembly during postovulatory aging and prevents deterioration in mouse oocyte quality. Spindle dynamics decreased with reduced TCTP level during aging of mouse oocytes. Knockdown of TCTP accelerated the reduction of spindle dynamics, accompanying with aging-related deterioration of oocyte quality. Conversely, overexpression of TCTP prevented aging-associated decline of spindle dynamics. Moreover, the aging-related abnormalities in oocytes were rescued after TCTP overexpression, thereby improving fertilization competency and subsequent embryo development. Therefore, our results demonstrate that TCTP-mediated spindle dynamics play a key role in maintaining oocyte quality during postovulatory aging and overexpression of TCTP is sufficient to prevent aging-associated abnormalities in mouse oocytes. |
| Databáze: | OpenAIRE |
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