Time-course changes in ectonucleotidase activities during experimental autoimmune encephalomyelitis
| Autor: | Mirjana Stojiljkovic, Ivana Bjelobaba, Marija Mostarica-Stojkovic, Nadezda Nedeljkovic, Sanja Pekovic, Stanislava Stosic-Grujicic, Irena Lavrnja |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Adenosine Encephalomyelitis Autoimmune Experimental Multiple Sclerosis Time Factors Encephalomyelitis Central nervous system Biology 03 medical and health sciences Cellular and Molecular Neuroscience Adenosine Triphosphate 0302 clinical medicine Blood serum Internal medicine Nucleotidase medicine Extracellular Animals Ectonucleotidase 5'-Nucleotidase 030304 developmental biology 0303 health sciences Apyrase Cell Membrane Experimental autoimmune encephalomyelitis Rats Inbred Strains Cell Biology medicine.disease Adenosine Monophosphate Rats Adenosine Diphosphate Disease Models Animal medicine.anatomical_structure Endocrinology Spinal Cord Immunology Female 030217 neurology & neurosurgery |
| Zdroj: | Neurochemistry International |
| ISSN: | 0197-0186 |
| DOI: | 10.1016/j.neuint.2009.02.013 |
| Popis: | The aim of the present study was to analyze the activities of extracellular purine metabolizing enzymes, CD39 (apyrase, EC 3.6.1.5) and CD73 (ecto-5' nucleotidase, EC 3.1.3.5) in experimental autoimmune encephalomyelitis (EAE). The levels of ATP, ADP and AMP hydrolysis were analyzed in the blood serum and in the rat spinal cord plasma membrane preparation 8, 15 and 25 days after induction of EAE. The animals were divided in three groups: control (saline), CFA (adjuvant-only) and EAE (CFA and homogenate of spinal cords). Eight days after immunization, ATP, ADP and AMP hydrolysis in the blood serum and spinal cord membrane preparations were unaffected in EAE compared to both, control and CFA group. In the peak of disease, ATP, ADP and AMP hydrolysis in EAE group showed significant decrease in the blood serum and prominent increase in the spinal cord membrane preparation compared to CFA and control group. At the end of illness, as judged by disappearance of clinical manifestation of EAE, ATP, ADP and AMP hydrolysis, although closer to CFA levels, were still significantly different in respect to the CFA group. Modulation of ATP, ADP and AMP hydrolysis suggests that they operate during EAE and might represent the basis of novel therapeutic strategies in immune-mediated diseases, such as MS. (C) 2009 Elsevier Ltd. All rights reserved. null |
| Databáze: | OpenAIRE |
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