Migratory birds as a potential overseas transmitter of Shiga toxin-producing Escherichia coli
| Autor: | Aya Seleem, Maha A. Sabry, Khaled A. Abdel-Moein |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
migratory birds
General Veterinary Phylogenetic tree medicine.drug_class animal diseases Antibiotics public health Biology medicine.disease_cause Isolation (microbiology) bacterial infections and mycoses Quail Microbiology Multiple drug resistance fluids and secretions STX2 biology.animal medicine bacteria Shiga toxin-producingE. coli Escherichia coli Gene Research Article |
| Zdroj: | International Journal of Veterinary Science and Medicine article-version (VoR) Version of Record |
| ISSN: | 2314-4599 2314-4580 |
| Popis: | Shiga toxin-producing Escherichia coli (STEC) has a great public health importance. This study was conducted to investigate the potential role of migratory birds in the transmission of STEC. For this purpose, cloacal swabs were collected from 349 migratory birds (209 ducks and 140 quails) from Damietta governorate, Egypt. The collected swabs were cultured for isolation of STEC using the STEC CHROMagar. STEC isolates were identified based on colonial characteristics, Gram’s stain, conventional biochemical tests and molecular detection of stx1, stx2 and eae genes. Positive isolates were serotyped and examined for their antibiotic susceptibility pattern. Furthermore, gene sequencing was performed for genes stx1and stx2. Of the examined birds, two STEC isolates were a obtained with an overall occurrence rate 0.57% (2/349), one isolate carried stx2 gene from a migratory quail 0.71% (1/140), and another isolate from a migratory duck carried stx1 gene 0.48% (1/209), whereas both isolates were negative for eae gene. Moreover, the duck isolate was serotyped O86, while the quail isolate was serotyped O125; both isolates were multidrug resistant. The phylogenetic analysis of the obtained stx1 and stx2 genes revealed high genetic relatedness to those isolated from human cases in the countries where such birds either lived or were in their migratory pathway. In conclusion, this study highlights the potential role of migratory birds in transmitting multidrug-resistant STEC across their migratory pathway. |
| Databáze: | OpenAIRE |
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