Imaging the dynamics of individual electropores
| Autor: | Mark I. Wallace, Jason T. Sengel |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Materials science Lipid Bilayers Nanotechnology 02 engineering and technology Materials testing Gating Applied potential Permeability Membrane Potentials 03 medical and health sciences Electromagnetic Fields Phase (matter) Materials Testing Lipid bilayer Multidisciplinary Dynamics (mechanics) Biological Sciences 021001 nanoscience & nanotechnology Kinetics Electroporation 030104 developmental biology Membrane Microscopy Fluorescence Chemical physics 0210 nano-technology Porosity |
| Zdroj: | Sengel, J T & Wallace, M I 2016, ' Imaging the dynamics of individual electropores ', Proceedings of the National Academy of Sciences, vol. 113, no. 19, pp. 5281-5286 . https://doi.org/10.1073/pnas.1517437113 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Electroporation is a widely used technique to permeabilize cell membranes. Despite its prevalence, our understanding of the mechanism of voltage-mediated pore formation is incomplete; methods capable of visualizing the time-dependent behavior of individual electropores would help improve our understanding of this process. Here, using optical single-channel recording, we track multiple isolated electropores in real time in planar droplet interface bilayers. We observe individual, mobile defects that fluctuate in size, exhibiting a range of dynamic behaviors. We observe fast (25 s(-1)) and slow (2 s(-1)) components in the gating of small electropores, with no apparent dependence on the applied potential. Furthermore, we find that electropores form preferentially in the liquid disordered phase. Our observations are in general supportive of the hydrophilic toroidal pore model of electroporation, but also reveal additional complexity in the interactions, dynamics, and energetics of electropores. |
| Databáze: | OpenAIRE |
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