| Autor: |
Ning-Xia, Xiong, Jin-Fang, Huang, Ke-Xin, Li, Shi-Yun, Li, Jia-Hui, Zhao, Rou, Wang, Jie, Ou, Lan-Fen, Fan, Sheng-Wei, Luo, Shao-Jun, Liu |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Fish & Shellfish Immunology. 126:197-210 |
| ISSN: |
1050-4648 |
| DOI: |
10.1016/j.fsi.2022.05.039 |
| Popis: |
Ferritin M is involved in the regulation of fish immunity. In this study, open reading frame (ORF) sequences of ferritin M from hybrid fish and its parental species were 534 bp. Tissue-specific analysis indicated that the highest level of ferritin M from red crucian carp was observed in kidney, while peaked expressions of ferritin M from white crucian carp and hybrid carp were observed in gill. Elevated levels of ferritin M from hybrid carp and its parental species were detected in immune-related tissues following Aeromonas hydrophila infection or in cultured fish cell lines after lipopolysaccharide (LPS) challenge. Ferritin M overexpression could attenuate NF-κB and TNFα promoter activity in their respective fish cells. Purified ferritin M fusion proteins elicited in vitro binding activity to A. hydrophila and Edwardsiella tarda, lowered bacterial dissemination to tissues and alleviated inflammatory response. Furthermore, treatment with ferritin M fusion proteins could mitigate bacteria-induced liver damage and rescue antioxidant activity. These results suggested that ferritin M in hybrid fish showed a similar immune defense against bacteria infection in comparison with those of its parental species. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect