Bone metabolism markers and angiogenic cytokines as regulators of human hematopoietic stem cell mobilization
| Autor: | Christina Kalpadakis, Gerassimos A. Pangalis, Pantelis Tsirkinidis, Konstantinos Konstantopoulos, Marina P. Siakantaris, Athanasios Papatheodorou, Marie-Christine Kyrtsonis, Theodoros P. Vassilakopoulos, Eleni Lalou, Aglaia Dimitrakopoulou, Maria K. Angelopoulou, Georgios Boutsikas, Evangelos Terpos, Konstantinos Anargyrou, Panayiotis Panayiotidis |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male medicine.medical_specialty Time Factors Adolescent Endocrinology Diabetes and Metabolism CD34 Neovascularization Physiologic Osteoclasts Bone and Bones Bone remodeling 03 medical and health sciences Young Adult 0302 clinical medicine Endocrinology Osteoprotegerin N-terminal telopeptide Internal medicine Bone cell medicine Humans Orthopedics and Sports Medicine Hematopoietic Stem Cell Mobilization Aged Osteoblasts biology RANK Ligand Hematopoietic stem cell General Medicine Middle Aged 030104 developmental biology medicine.anatomical_structure RANKL 030220 oncology & carcinogenesis biology.protein Cytokines Female Biomarkers |
| Zdroj: | Journal of bone and mineral metabolism. 36(4) |
| ISSN: | 1435-5604 |
| Popis: | Hematopoietic stem cell (HSC) mobilization involves cleavage of ligands between HSC and niche components. However, there are scarce data regarding the role of bone cells in human HSC mobilization. We studied biochemical markers of bone metabolism and angiogenic cytokines during HSC mobilization in 46 patients' sera with lymphoma and multiple myeloma, by ELISA. Significant changes between pre-mobilization and collection samples were found: (1) Bone alkaline phosphatase (BALP) increased, indicating augmentation of bone formation; (2) Receptor activator of Nf-κB ligand/osteoprotegerin ratio (RANKL/OPG) increased, showing osteoclastic differentiation and survival; however, there was no evidence of increased osteoclastic activity; and (3) Angiopoietin-1/Angiopoietin-2 ratio (ANGP-1/ANGP-2) decreased, consistent with vessel destabilization. Poor mobilizers had significantly higher carboxy-terminal telopeptide of collagen type I (CTX) and lower ANGP-1 at pre-mobilization samples, compared to good ones. CTX, amino-terminal telopeptide of collagen type I (NTX) and ANGP-1 pre-mobilization levels correlated significantly with circulating CD34+ peak cell counts. Our results indicate that bone formation and vessel destabilization are the two major events during human HSC mobilization. Osteoblasts seem to be the orchestrating cells, while osteoclasts are stimulated but not fully active. Moreover, ANGP-1, CTX and NTX may serve as predictors of poor mobilization. |
| Databáze: | OpenAIRE |
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