Glucose uptake by adipocytes of obese rats: effect of one bout of acute exercise
| Autor: | Y. Zick-Bachar, R. Burstein, E. Karnieli, A. Zissholtz, Yair Shapiro, Y. Epstein |
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| Rok vydání: | 1992 |
| Předmět: |
Blood Glucose
Male Glucose Clearance medicine.medical_specialty Physiology medicine.medical_treatment Glucose uptake Physical Exertion Carbohydrate metabolism Biological effect Rats Sprague-Dawley Cellular mechanism Physiology (medical) Internal medicine medicine Animals Insulin Obesity Rest (music) Epididymis Pharmacology Chemistry Insulin dosage Methylglucosides Biological Transport General Medicine Rats Kinetics Glucose Endocrinology Adipose Tissue 3-O-Methylglucose |
| Zdroj: | Canadian Journal of Physiology and Pharmacology. 70:1473-1476 |
| ISSN: | 1205-7541 0008-4212 |
| Popis: | The effect of one bout of acute exercise on impaired glucose metabolism was studied in obese (480 ± 20 g), untrained rats, at rest (n = 10) and after 60 min of swimming (n = 5). Using the euglycemic, hyperinsulinemic (10 mU∙kg−1∙min−1) clamp, glucose clearance rate increased from 7.6 ± 0.9 at rest to 9.7 ± 0.5 mL∙kg−1∙min−1 after exercise (p 14C]methylglucose) transport (GT) into epididymal adipocytes was also studied. Animals were sacrificed at rest (n = 21) or after swimming (n = 18) and their epididymal adipocytes were incubated with or without insulin. In the absence of insulin, GT was 0.13 ± 0.02 and 0.26 ± 0.07 fmol∙cell−1∙min−1 at rest and after exercise, respectively. In the presence of insulin (25–1000 μU∙mL−1) GT increased at rest from 0.97 ± 0.08 to 1.13 ± 0.07 fmol∙cell−1∙min−1, and after exercise from 1.35 ± 0.05 to 1.87 ± 0.11 fmol∙cell−1∙min−1. GT was significantly higher after exercise compared with rest (p −1, whereas with exercise, GT increased gradually with the insulin dosage. The following may be concluded: (i) the biological effect of insulin is amplified in obese rats by one bout of exercise and (ii) exercise affects GT into enlarged adipocytes by enhancing tissue responsiveness to insulin and by a cellular mechanism unrelated to the insulin action.Key words: glucose uptake, euglycemic clamp, glucose transport, obesity, exercise. |
| Databáze: | OpenAIRE |
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