A novel sodium overload test predicting ascites decompensation in rats with CCl4-induced cirrhosis
| Autor: | Marco Domenicali, Anna Maria Pertosa, Paolo Caraceni, Wladimiro Jiménez, Pasquale Chieco, Josefa Ros, A. Principe, Mauro Bernardi, Franco Trevisani |
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| Přispěvatelé: | Domenicali M., Caraceni P., Principe A., Pertosa A.M., Ros J., Chieco P., Trevisani F., Jimenez W., Bernardi M. |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Male
medicine.medical_specialty Cirrhosis Sodium chemistry.chemical_element Urine Sodium Chloride Liver Cirrhosis Experimental Gastroenterology chemistry.chemical_compound SODIUM OMEOSTASIS Oral administration Internal medicine Ascites Medicine Animals Homeostasis Decompensation Rats Wistar Carbon Tetrachloride Hepatology Dose-Response Relationship Drug business.industry Hemodynamics SODIUM OVERLOAD TEST medicine.disease Survival Analysis Rats Endocrinology chemistry Effusion EXPERIMENTAL LIVER CIRRHOSIS Carbon tetrachloride ASCITES medicine.symptom business |
| Popis: | Background/Aims We aimed to develop a non-invasive test to identify the initial alterations of sodium homeostasis and prospectively predict decompensation in preascitic cirrhotic rats. Methods The sodium overload test (SOT) was performed in control (CT) and CCl 4 -induced cirrhotic rats (CH) by calculating the percentage of sodium excreted in the urine after NaCl oral administration (0.5g/kg). Liver fibrosis was quantified by image cytometry. Results From the 8th week of CCl 4 intoxication, while the daily sodium balance did not change in CH and CT, SOT became significantly lower in the former (62.1±13.2 vs 78.8±13.2%; P =0.035). At sacrifice, ascites was only present in one animal. The degree of liver fibrosis correlated with SOT. In subsequent experiments, 17 cirrhotic rats developed ascites between the 9th and 14th weeks. SOT remained stable up to 3 weeks before ascites appearance, while it fell significantly to 35±19 and 26±21% at 2 and 1 week before ascites diagnosis, respectively. Nearly all the rats (95%) with a SOT Conclusions In preascitic cirrhotic rats, SOT unveils sodium metabolism abnormalities earlier than the daily sodium balance and prospectively predicts ascites appearance, identifying rats in a homogeneous stage of cirrhosis, which is essential in pathophysiological studies on sodium retention. |
| Databáze: | OpenAIRE |
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