High-Affinity K+ Transporters from a Halophyte,Sporobolus virginicus, Mediate Both K+ and Na+ Transport in Transgenic Arabidopsis,X. laevisOocytes and Yeast
| Autor: | Maki Katsuhara, Yuichi Tada, Takamitsu Kurusu, Mineo Shibasaka, Yoshiki Nakahara, Tomoaki Horie, Chisato Endo |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Mutant Arabidopsis Xenopus Saccharomyces cerevisiae Plant Science Phloem Genes Plant Poaceae Plant Roots 01 natural sciences Xenopus laevis 03 medical and health sciences Gene Expression Regulation Plant Xylem Halophyte Tobacco Animals Transgenes Cation Transport Proteins Phylogeny Ions biology Chemistry Sodium fungi food and beverages Biological Transport Salt-Tolerant Plants Cell Biology General Medicine Plants Genetically Modified biology.organism_classification Yeast 030104 developmental biology Oocytes Potassium Biophysics Sporobolus virginicus Homeostasis 010606 plant biology & botany |
| Zdroj: | Plant and Cell Physiology. 60:176-187 |
| ISSN: | 1471-9053 0032-0781 |
| DOI: | 10.1093/pcp/pcy202 |
| Popis: | Class II high-affinity potassium transporters (HKTs) have been proposed to mediate Na+-K+ co-transport in plants, as well as Na+ and K+ homeostasis under K+-starved and saline environments. We identified class II HKTs, namely SvHKT2;1 and SvHKT2;2 (SvHKTs), from the halophytic turf grass, Sporobolus virginicus. SvHKT2;2 expression in S. virginicus was up-regulated by NaCl treatment, while SvHKT2;1 expression was assumed to be up-regulated by K+ starvation and down-regulated by NaCl treatment. Localization analysis revealed SvHKTs predominantly targeted the plasma membrane. SvHKTs complemented K+ uptake deficiency in mutant yeast, and showed both inward and outward K+ and Na+ transport activity in Xenopus laevis oocytes. When constitutively expressed in Arabidopsis, SvHKTs mediated K+ and Na+ accumulation in shoots under K+-starved conditions, and the K+ concentration in xylem saps of transformants was also higher than in those of wild-type plants. These results suggest transporter-enhanced K+ and Na+ uploading to the xylem from xylem parenchyma cells. Together, our data demonstrate that SvHKTs mediate both outward and inward K+ and Na+ transport in X. laevis oocytes, and possibly in plant and yeast cells, depending on the ionic conditions. |
| Databáze: | OpenAIRE |
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