Outer Membrane Proteins and Serosubtyping with Outer Membrane Vesicles from Clinical Isolates of Neisseria meningitidis
| Autor: | James W. Coulton, Mills El, Arhin Ff, Moreau F |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Serotype
medicine.drug_class Porins Enzyme-Linked Immunosorbent Assay Neisseria meningitidis Monoclonal antibody medicine.disease_cause Applied Microbiology and Biotechnology Microbiology medicine Typing Serotyping biology Vesicle Cell Membrane Quebec General Medicine biology.organism_classification Virology Membrane protein Genes Bacterial Neisseriaceae Bacterial outer membrane Bacterial Outer Membrane Proteins |
| Zdroj: | Current Microbiology. 34:18-22 |
| ISSN: | 1432-0991 0343-8651 |
| DOI: | 10.1007/s002849900137 |
| Popis: | The currently practiced protocol for routine serosubtyping of Neisseria meningitidis relies on reactivity of whole cells to monoclonal antibodies against the class 1 outer membrane protein (OMP) in ELISAs or dot-blots. This procedure, however, failed to yield serosubtyping information in 28% (48/174) of clinical isolates (1993-1994) in the province of Québec, Canada. These 48 strains were characterized by OMP profiles and ELISAs with outer membrane vesicles (OMVs). Forty out of the 48 strains expressed class 1 OMP, indicating that the inability to assign a serosubtype was not owing to the absence of the class 1 OMP. Of these, 15 (38%) were serosubtypable in ELISAs with outer membrane vesicles. Thus, 81% (141/174) of all meningococcal strains were serosubtypable with ELISAs using whole-cells or OMVs. Because the routinely used procedure for serosubtyping of meningococci is limited in providing serosubtype information, alternate procedures are proposed to obtain comprehensive information for epidemiological identification of this bacterium. |
| Databáze: | OpenAIRE |
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