Characterization of an Axenic Strain ofHartmannella vermiformisObtained from an Investigation of Nosocomial Legionellosis

Autor:	Thomas K. Sawyer, C. Harold King, Gary N. Sanden, William T. Martin, W E Morrill, J M Barbaree, Barry S. Fields, Thomas A. Nerad
Rok vydání:	1990
Předmět:	Cross Infection Hartmannella Legionellosis biology biology.organism_classification Pathogenicity Legionella pneumophila Hospitals Hartmannella vermiformis Water sample Disease Outbreaks Microbiology Isoenzymes Water Supply Animals Germ-Free Life Humans Parasitology Axenic Axenic culture Bacteria
Zdroj:	The Journal of Protozoology. 37:581-583
ISSN:	0022-3921
Popis:	A free-living amoeba identified as Hartmannella vermiformis was isolated from a water sample obtained during an investigation of nosocomial legionellosis. Hartmannella vermiformis is known to support the intracellular multiplication of Legionella pneumophila. This strain of H. vermiformis, designated CDC-19, was cloned and established in axenic culture to develop a model for the study of the pathogenicity of legionellae. Isoenzyme patterns of axenically-cultivated strain CDC-19 were compared with two strains of H. vermiformis derived from the type strain, one axenic (ATCC 50236) and the other grown in the presence of bacteria (ATCC 30966). Enzyme patterns suggested that all three strains are assignable to the species H. vermiformis. Axenic H. vermiformis strain CDC-19 has been deposited with the American Type Culture Collection (ATCC 50237) and should prove useful in the study of protozoan-bacterial interaction.
Databáze:	OpenAIRE
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