Thin magnesium layer confirmed as an antibacterial and biocompatible implant coating in a co‑culture model
| Autor: | Sarah Zaatreh, Eckhard Quandt, Madlen Strauss, David Haffner, Rainer Bader, Thomas Dauben, Christiane Zamponi, Bernd Kreikemeyer, Wolfram Mittelmeier |
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| Rok vydání: | 2016 |
| Předmět: |
Male
Cancer Research Pathology implant-associated infections 02 engineering and technology Biochemistry Coating Coated Materials Biocompatible Staphylococcus epidermidis human osteoblasts Magnesium Colony-forming unit Titanium biology Prostheses and Implants Articles Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Plankton Anti-Bacterial Agents Oncology fast corrosion surface Molecular Medicine Female 0210 nano-technology medicine.medical_specialty titanium alloy Cell Survival 0206 medical engineering chemistry.chemical_element engineering.material Models Biological magnesium coating Genetics medicine Alloys Humans Molecular Biology Aged Ions Osteoblasts Biofilm Titanium alloy biology.organism_classification 020601 biomedical engineering co-culture Coculture Techniques Culture Media chemistry engineering Implant Nuclear chemistry |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 |
| Popis: | Implant-associated infections commonly result from biofilm‑forming bacteria and present severe complications in total joint arthroplasty. Therefore, there is a requirement for the development of biocompatible implant surfaces that prevent bacterial biofilm formation. The present study coated titanium samples with a thin, rapidly corroding layer of magnesium, which were subsequently investigated with respect to their antibacterial and cytotoxic surface properties using a Staphylococcus epidermidis (S. epidermidis) and human osteoblast (hOB) co‑culture model. Primary hOBs and S. epidermidis were co‑cultured on cylindrical titanium samples (Ti6Al4V) coated with pure magnesium via magnetron sputtering (5 µm thickness) for 7 days. Uncoated titanium test samples served as controls. Vital hOBs were identified by trypan blue staining at days 2 and 7. Planktonic S. epidermidis were quantified by counting the number of colony forming units (CFU). The quantification of biofilm‑bound S. epidermidis on the surfaces of test samples was performed by ultrasonic treatment and CFU counting at days 2 and 7. The number of planktonic and biofilm‑bound S. epidermidis on the magnesium‑coated samples decreased by four orders of magnitude when compared with the titanium control following 7 days of co‑culture. The number of vital hOBs on the magnesium‑coated samples was observed to increase (40,000 cells/ml) when compared with the controls (20,000 cells/ml). The results of the present study indicate that rapidly corroding magnesium‑coated titanium may be a viable coating material that possesses antibacterial and biocompatible properties. A co‑culture test is more rigorous than a monoculture study, as it accounts for confounding effects and assesses additional interactions that are more representative of in vivo situations. These results provide a foundation for the future testing of this type of surface in animals. |
| Databáze: | OpenAIRE |
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