Interleukin-10 protects against blood-induced joint damage
| Autor: | Michel J. J. Hooiveld, Goris Roosendaal, N.W. Jansen, Matthias Theobald, Joel A G van Roon, Floris P J G Lafeber, Johannes W. J. Bijlsma |
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| Rok vydání: | 2008 |
| Předmět: |
musculoskeletal diseases
Cartilage Articular Male Pathology medicine.medical_specialty Necrosis Erythrocytes Interleukin-1beta Drug Evaluation Preclinical Hemophilia A Peripheral blood mononuclear cell Proinflammatory cytokine Tissue Culture Techniques In vivo Internal medicine Hemarthrosis medicine Humans Hematology business.industry Tumor Necrosis Factor-alpha Cartilage Synovial Membrane medicine.disease Interleukin-10 Interleukin 10 medicine.anatomical_structure Blood Leukocytes Mononuclear Proteoglycans medicine.symptom business |
| Zdroj: | British journal of haematology. 142(6) |
| ISSN: | 1365-2141 |
| Popis: | Summary Despite prophylactic treatment, haemophilia patients suffer from spontaneous joint bleeds, which lead to severe joint damage. Also after joint trauma, an intra-articular haemorrhage can add to joint damage over time. This study evaluated interleukin 10 (IL-10) in the search for possible interventions to prevent or limit the damaging effects of joint bleeds. Human articular cartilage tissue explants were cultured in the presence or absence of 50% v/v blood (or its cellular components) for 4 d (the expected blood load in vivo after a joint haemorrhage), followed by a recovery period of 12 d. Pharmacological dosages of IL-10 reached during treatment (1 or 10 ng/ml) were added. Additionally, cartilage and synovial tissue obtained from joints with end-stage haemophilic arthropathy (HA) were cultured in the presence of IL-10 (10 ng/ml). IL-10 protected cartilage from the damaging effects of blood exposure, measured by its effects on proteoglycan turnover. In addition, IL-10 beneficially influenced cartilage from patients with HA and reduced the production of the inflammatory cytokines IL-1β and tumour necrosis factor-α by haemophilic synovial tissue. Taken together, although effects were obtained in vitro, IL-10 protects against blood-induced joint damage and might be further evaluated as candidate in treatment of tissue damaging effects of joint haemorrhages. |
| Databáze: | OpenAIRE |
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