The first extracellular domain of the tumour stem cell marker CD133 contains an antigenic ganglioside-binding motif

Autor: Xiao-Jun Guo, Nouara Yahi, Nicolas Garmy, Marc Maresca, Nadira Taïeb, Jacques Fantini
Přispěvatelé: Centre de recherche en neurobiologie - neurophysiologie de Marseille (CRN2M), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre d'Immunologie de Marseille - Luminy (CIML), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Institut méditerranéen de Recherche en Nutrition : Biochimie et Biologie de la Nutrition (IMRN), Université Paul Cézanne - Aix-Marseille 3-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Université Paul Cézanne - Aix-Marseille 3-Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Cancer Research
MESH: HT29 Cells
MESH: Membrane Microdomains
MESH: Amino Acid Sequence
Stem cell marker
Epitope
MESH: Protein Structure
Tertiary
0302 clinical medicine
Complementary
Gangliosides
AC133 Antigen
Tumor Markers
Lipid raft
MESH: Antigens
CD
0303 health sciences
MESH: Peptides
MESH: G(M1) Ganglioside
CD
3. Good health
Cell biology
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biomolecules [q-bio.BM]
Oncology
030220 oncology & carcinogenesis
Colonic Neoplasms
embryonic structures
Neoplastic Stem Cells
lipids (amino acids
peptides
and proteins)
MESH: Caco-2 Cells
Stem cell
HT29 Cells
Protein Structure
DNA
Complementary
Molecular Sequence Data
MESH: Glycoproteins
G(M1) Ganglioside
Biology
03 medical and health sciences
Membrane Microdomains
Antigens
CD
Ganglioside binding
Cancer stem cell
Biomarkers
Tumor
Humans
Amino Acid Sequence
Antigens
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
neoplasms
Glycoproteins
030304 developmental biology
MESH: Gangliosides
MESH: Colonic Neoplasms
Binding Sites
Ganglioside
MESH: Humans
MESH: Molecular Sequence Data
DNA
MESH: DNA
Complementary
Biological
Molecular biology
MESH: Neoplastic Stem Cells
Protein Structure
Tertiary
carbohydrates (lipids)
MESH: Binding Sites
MESH: Tumor Markers
Biological
Caco-2 Cells
Peptides
Tertiary
Zdroj: Cancer Letters
Cancer Letters, Elsevier, 2009, 278 (2), pp.164-73. ⟨10.1016/j.canlet.2009.01.013⟩
Cancer Letters, 2009, 278 (2), pp.164-73. ⟨10.1016/j.canlet.2009.01.013⟩
ISSN: 0304-3835
Popis: International audience; Prominin 1/CD133 is a marker of transplantable cancer stem cells. We have generated anti-peptide antibodies against a N-terminal epitope of CD133 belonging to a ganglioside-binding domain. The labelling of colon cancer cells with these antibodies was inhibited by various gangliosides including GM1 and GD3, but not GT1b. CD133 immunolabelling progressively decreased to undetectable levels in post-confluent cultures, possibly through ganglioside-mediated epitope masking since the staining was partially recovered after chemical disruption of lipid rafts. We suggest that selected gangliosides could modulate the accessibility of CD133 and regulate cell-cell contacts involving CD133(+) stem cells at the earliest steps of tumour development.
Databáze: OpenAIRE
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