Ion Channels Induced by Antimicrobial Agents in Model Lipid Membranes are Modulated by Plant Polyphenols Through Surrounding Lipid Media
Autor: | Olga S. Ostroumova, Svetlana S. Efimova, Roman Ya. Medvedev, Anastasiia A. Zakharova |
---|---|
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Naringenin Licochalcone A Physiology Membrane lipids Lipid Bilayers Biophysics Ion Channels Membrane Lipids 03 medical and health sciences chemistry.chemical_compound Chalcones 0302 clinical medicine Anti-Infective Agents Lipid bilayer Ion channel Butein Bilayer Polyphenols food and beverages Cell Biology 030104 developmental biology Membrane chemistry 030220 oncology & carcinogenesis Flavanones |
Zdroj: | The Journal of Membrane Biology. 251:551-562 |
ISSN: | 1432-1424 0022-2631 |
Popis: | The potential therapeutic applications of plant polyphenols in various neurological, cardiovascular, metabolic and malignant disorders determine the relevance of studying the molecular mechanisms of their action on the cell membranes. Here, the quantitative changes in the physical parameters of model bilayer lipid membranes upon the adsorption of plant polyphenols were evaluated. It was shown that butein and naringenin significantly decreased the intrinsic dipole potential of cholesterol-free and cholesterol-enriched membranes. Cardamonin, 4'-hydroxychalcone, licochalcone A and liquiritigenin demonstrated the average efficiency, while resveratrol did not characterized by the ability to modulate the bilayer electrostatics. At the same time, the tested polyphenols affected melting of phospholipids with saturated acyl chains. The effects were attributed to the lipid disordering and a promotion of the positive curvature stress. According to DSC data and results of measurements of the threshold voltages that cause bilayer breakdown licochalcone A is the most effective agent. Furthermore, the role of the polyphenol induced changes in the electric and elastic properties of lipid host in the regulation of reconstituted ion channels was examined. The ability of the tested polyphenols to decrease the conductance of single ion channels produced by the antifungal cyclic lipopeptide syringomycin E was in agreement with their effects on the dipole potential of the lipid bilayers. The greatest effect of licochalcone A on the steady-state membrane conductance induced by the antifungal polyene macrolide antibiotic nystatin correlated with its greatest efficacy to induce the positive curvature stress. We also found that butein and naringenin bind specifically to a single pore formed by α-hemolysin from Staphylococcus aureus. |
Databáze: | OpenAIRE |
Externí odkaz: |