Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of Legionella pneumophila and other Legionella species
| Autor: | Kate Templeton, Herman Goossens, Sitha A. Scheltinga, Jantine W. Crielaard, Peter Sillekens, Eric C. J. Claas, Alje P. van Dam |
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| Přispěvatelé: | Academic Medical Center |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Microbiology (medical)
biology Legionella Reproducibility of Results Bacteriology biology.organism_classification bacterial infections and mycoses Polymerase Chain Reaction Sensitivity and Specificity Legionella pneumophila Virology Microbiology respiratory tract diseases Single tube Real-time polymerase chain reaction Multiplex polymerase chain reaction Humans bacteria Multiplex Human medicine Legionella species Clinical evaluation |
| Zdroj: | Journal of clinical microbiology, 41(9), 4016-4021. American Society for Microbiology Journal of clinical microbiology |
| ISSN: | 0095-1137 |
| Popis: | A multiplex real-time PCR assay for detection of Legionella pneumophila and Legionella spp. and including an internal control was designed. Legionella species, L. pneumophila , and the internal control were detected simultaneously by probes labeled with 6-carboxy-fluorescein, hexachlorofluorescein, and indodicarbocyanine, respectively. Therefore, no postamplification analysis was required in order to distinguish the targets. The sensitivity of both assays was 2.5 CFU/ml, and from analysis of 10 culture-positive and 74 culture-negative samples from patients investigated for legionellosis, 100% agreement was observed by both assays in comparison to culture. Four additional positives were found by the multiplex real-time PCR assay in the Legionella culture-negative samples. |
| Databáze: | OpenAIRE |
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