Water Sampling Module for Collecting and Concentrating Legionella pneumophila from Low-to-Medium Contaminated Environment
| Autor: | Jan J. Dubowski, Lydston Rodrigues de Carvalho, Khalid Moumanis, Lilian Sirbu, Pierre Hiernaux, Walid M. Hassen, Eric Frost |
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| Přispěvatelé: | Laboratoire Nanotechnologies et Nanosystèmes [Sherbrooke] (LN2), Université de Sherbrooke (UdeS)-École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Institut Interdisciplinaire d'Innovation Technologique [Sherbrooke] (3IT), Université de Sherbrooke (UdeS) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
water samples
0301 basic medicine Legionella pneumophila lcsh:Biotechnology [SDV]Life Sciences [q-bio] 030106 microbiology Clinical Biochemistry Backwashing 010501 environmental sciences cooling towers 01 natural sciences Article law.invention 03 medical and health sciences [SPI]Engineering Sciences [physics] Saline solutions law lcsh:TP248.13-248.65 Concentration factor concentration factor bacteria Filtration Water sampling ComputingMilieux_MISCELLANEOUS 0105 earth and related environmental sciences Waste management biology filtration system Water Pollution Water General Medicine Contamination biology.organism_classification 6. Clean water Contaminated water recovery rate Environmental science Water Microbiology Legionnaires’ disease Environmental Monitoring |
| Zdroj: | Biosensors Biosensors, Elsevier, 2021, 11 (2), pp.34. ⟨10.3390/bios11020034⟩ Biosensors, Vol 11, Iss 34, p 34 (2021) Volume 11 Issue 2 |
| ISSN: | 0265-928X |
| DOI: | 10.3390/bios11020034⟩ |
| Popis: | The detection of water contamination with Legionella pneumophila is of critical importance to manufacturers of water processing equipment and public health entities dealing with water networks and distribution systems. Detection methods based on polymerase chain reaction or biosensor technologies require preconcentration steps to achieve attractive sensitivity levels. Preconcentration must also be included in protocols of automated collection of water samples by systems designed for quasi-continuous monitoring of remotely located water reservoirs for the presence of L. pneumophila. We designed and characterized a water sampling module for filtration and backwashing intended for analysis of low-to-medium contaminated water, typically with L. pneumophila bacteria not exceeding 50 colony-forming units per milliliter. The concentration factors of 10× and 21× were achieved with 0.22 and 0.45 µm filters, respectively, for samples of bacteria prepared in clean saline solutions. However, a 5× concentration factor was achieved with 0.45 µm filters for a heavily contaminated or turbid water typical of some industrial water samples. |
| Databáze: | OpenAIRE |
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