An optimized procedure for whole-mount in situ hybridization on mouse embryos and embryoid bodies
| Autor: | Denis Piette, Erik Willems, Caroline R. Kemp, Luc Leyns, Marijke Hendrickx |
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| Přispěvatelé: | Biology, Cell Genetics |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
In situ
Gene Expression Profiling Gene Expression embryo Embryo In situ hybridization Computational biology Embryoid body Biology Embryo Mammalian Embryonic stem cell General Biochemistry Genetics and Molecular Biology RNA Complementary Cell biology Mice Embryology embryonic structures Animals whole-mount in situ hybridization protocol DNA microarray Developmental biology |
| Zdroj: | Vrije Universiteit Brussel |
| Popis: | Determining the precise expression pattern of a gene of interest at various stages of development is essential to understanding its biological function in embryology. This protocol describes a sensitive method for whole-mount in situ hybridization (WISH) to mouse embryos, using cRNA probes. Adaptations are provided that allow the protocol to be applied to embryonic stages ranging from blastocysts to postimplantation stage embryos, and to embryoid bodies. We also describe an in situ method for differential detection of two probes. Probe labeling and dissection and preparation of the embryos can be performed in 2 d. The actual WISH procedure can be completed in another 3 d. |
| Databáze: | OpenAIRE |
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