Poly I:C/Poly-L-Lysine: Potent Inducer of Interferons in Primates
| Autor: | Maurice R. Hilleman, Lampson Gp, Alfred A. Tytell, A. Kirk Field |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Interferon Inducers
Immunology Lysine Biology complex mixtures Interferon Virology Chlorocebus aethiops medicine Animals Polylysine Nucleotide Inducer I²C chemistry.chemical_classification Molecular mass Potent inducer Molecular Weight Drug Combinations Titer Poly I-C Solubility Biochemistry chemistry bacteria Peptides medicine.drug |
| Zdroj: | Journal of Interferon Research. 1:539-549 |
| ISSN: | 0197-8357 |
| Popis: | A soluble complex of poly I:C and poly-L-lysine (poly I:C/poly-L-lysine) has been prepared that induces high titers of circulating interferon in monkeys. By limiting the molar ratio of lysine to nucleotide to 0.5, a complex was formed that was soluble up to 2.0 mg poly I:C/ml of phosphate-buffered saline. Complexes of poly I:C with poly-L-lysine of various molecular weights, and in a constant ratio (0.5) of lysine to nucleotide, were evaluated for capacity to induce serum interferon in grivet monkeys. Substantial enhancement (10- to 100-fold) of the capacity of poly I:C to induce interferon in grivet monkeys was observed using poly I:C complexed with poly-L-lysines of molecular weight 10(4) daltons or greater. The poly I:C/poly-L-lysine as an effective inducer of interferon in grivet monkeys, rhesus monkeys, chimpanzees and marmosets. A high interferon titer (greater than 100 units/ml blood) was maintained in grivet monkeys by repeated daily administration of the complex. No long-term hyporesponsiveness was noted following repeat inductions over a period of months. The serum interferon produced in monkeys in response to poly I:C/poly-L-lysine resembled human leukocyte interferon in its biological characteristics. |
| Databáze: | OpenAIRE |
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