Gelatin Sponge as an Anchorage for Three-dimensional Culture of Colorectal Cancer Cells
| Autor: | Mayumi Nomura, Kazunari Sugimitsu, Muneyuki Koyama, Emi Kikuchi, G O Kuwata, Yuki Takemura, Hideki Saito, Toshiaki Tachibana, Yoshinobu Manome, Erika Osada, Keiichi Ikeda, Nobutake Akiyama |
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| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
Cell Survival Colorectal cancer Cell Culture Techniques Cell Movement Cell Line Tumor Cell Adhesion medicine Humans Phosphorylation Adaptor Proteins Signal Transducing Cell Proliferation Gelatin sponge Tissue Scaffolds biology Chemistry Cancer YAP-Signaling Proteins General Medicine Adhesion HCT116 Cells medicine.disease biology.organism_classification Molecular biology Sponge Oncology Transmission electron microscopy Cell culture Gelatin Immunohistochemistry Colorectal Neoplasms Transcription Factors |
| Zdroj: | Anticancer Research. 41:4259-4269 |
| ISSN: | 1791-7530 0250-7005 |
| DOI: | 10.21873/anticanres.15230 |
| Popis: | BACKGROUND Compared to two-dimensional cultures, three-dimensional (3D) cultures have many advantages in cancer studies. Nevertheless, their implementation is unsatisfactory. This study aimed to develop an anchorage-dependent 3D culture model for colorectal cancer research. MATERIALS AND METHODS Human HCT116, DLD-1 and SW620 colorectal cell lines were cultured in a gelatin sponge, and its applicability for morphological examination was studied. RESULTS The resulting specimens were suitable for scanning electron microscopy, transmission electron microscopy, and immunohistochemical examination. HCT116 formed smaller structures and migrated through the pores of the sponge. DLD-1 formed larger structures with tight cell-to-cell adhesion. SW620 also formed large structures but small clustered cells tended to attach to the anchorage more favorably. Immunohistochemical staining demonstrated phosphorylated yes-associated protein (YAP) localized near the attachment site in HCT116 cells. CONCLUSION Because the gelatin sponge provided suitable anchorage and the cultured cells formed distinguishable 3D structures, this method may be useful for further colorectal cancer research. |
| Databáze: | OpenAIRE |
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