Eosinophil adhesion to cholinergic nerves via ICAM-1 and VCAM-1 and associated eosinophil degranulation
| Autor: | Emma Court, Richard W. Costello, Deborah A. Sawatzky, W. Graham McLean, Bharathy Kumaravel, David B. Jacoby, Paul J. Kingham, Allison D. Fryer |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Pulmonary and Respiratory Medicine
Integrins Physiology Guinea Pigs Intercellular Adhesion Molecule-1 Receptors Lymphocyte Homing Vascular Cell Adhesion Molecule-1 Integrin alpha4beta1 Biology Peptides Cyclic Cell Degranulation Rats Sprague-Dawley chemistry.chemical_compound Parasympathetic nervous system Parasympathetic Nervous System Physiology (medical) Cell Adhesion medicine Animals Humans Eosinophil degranulation Enzyme Inhibitors VCAM-1 Cholinergic neuron Cells Cultured Protein Kinase C Neurons Eosinophil cationic protein Dose-Response Relationship Drug Heparin Degranulation Antibodies Monoclonal Cell Biology Eosinophil Immunohistochemistry Acetylcholine Leukotriene C4 Rats Eosinophils medicine.anatomical_structure chemistry Immunology Female |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 282:L1279-L1288 |
| ISSN: | 1522-1504 1040-0605 |
| DOI: | 10.1152/ajplung.00279.2001 |
| Popis: | In vivo, eosinophils localize to airway cholinergic nerves in antigen-challenged animals, and inhibition of this localization prevents antigen-induced hyperreactivity. In this study, the mechanism of eosinophil localization to nerves was investigated by examining adhesion molecule expression by cholinergic nerves. Immunohistochemical and functional studies demonstrated that primary cultures of parasympathetic nerves express vascular cell adhesion molecule-1 (VCAM-1) and after cytokine pretreatment with tumor necrosis factor-α and interferon-γ intercellular adhesion molecule-1 (ICAM-1). Eosinophils adhere to these parasympathetic neurones after cytokine pretreatment via a CD11/18-dependent pathway. Immunohistochemistry and Western blotting showed that a human cholinergic nerve cell line (IMR-32) expressed VCAM-1 and ICAM-1. Inhibitory experiments using monoclonal blocking antibodies to ICAM-1, VCAM-1, or CD11/18 and with the very late antigen-4 peptide inhibitor ZD-7349 showed that eosinophils adhered to IMR-32 cells via these adhesion molecules. The protein kinase C signaling pathway is involved in this process as a specific inhibitor-attenuated adhesion. Eosinophil adhesion to IMR-32 cells was associated with the release of eosinophil peroxidase and leukotriene C4. Thus eosinophils adhere to cholinergic nerves via specific adhesion molecules, and this leads to eosinophil activation and degranulation; this may be part of the mechanism of eosinophil-induced vagal hyperreactivity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|