Inorganic polyphosphate enhances radio-sensitivity in a human non–small cell lung cancer cell line, H1299
| Autor: | Yusuke Matsuya, Sagiri Fukura, Hisashi Nakano, Manami Tamura, Kaori Tsutsumi, Hiroyuki Date, Tomoki Sugahara, Satoshi Sawada |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Radiation-Sensitizing Agents Cell Survival DNA repair Mitochondrion Radiation Tolerance 03 medical and health sciences chemistry.chemical_compound Adenosine Triphosphate 0302 clinical medicine Polyphosphates Carcinoma Non-Small-Cell Lung Cell Line Tumor medicine Humans DNA Breaks Double-Stranded Neoplasm Metastasis RC254-282 Chemistry X-Rays Polyphosphate Neoplasms. Tumors. Oncology. Including cancer and carcinogens General Medicine Combined Modality Therapy Adenosine Cell biology 030104 developmental biology Biochemistry Inorganic Chemicals Cell culture 030220 oncology & carcinogenesis Cancer cell Energy Metabolism Adenosine triphosphate DNA medicine.drug |
| Zdroj: | Tumor Biology, Vol 39 (2017) |
| ISSN: | 1423-0380 |
| Popis: | Inorganic polyphosphate is a linear polymer containing tens to hundreds of orthophosphate residues linked by high-energy phosphoanhydride bonds. Polyphosphate has been recognized as a potent anti-metastasis reagent. However, the molecular mechanism underlying polyphosphate action on cancer cells is poorly understood. In this study, we investigated the involvement of polyphosphate in radio-sensitivity using a human non-small cell lung cancer cell line, H1299. We found that polyphosphate treatment decreases cellular adenosine triphosphate levels, suggesting a disruption of energy metabolism. We also found that the induction of DNA double-strand breaks was enhanced in polyphosphate-treated cells after X-ray irradiation and colony formation assay revealed that cell survival decreased compared with that of the control groups. These findings suggest that polyphosphate is a promising radio-sensitizer for cancer cells. Therefore, we hypothesized that polyphosphate treatment disrupts adenosine triphosphate-mediated energy transfer for cellular survival and DNA repair, thereby reducing the cellular capability to resist X-ray irradiation. |
| Databáze: | OpenAIRE |
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