E12 technique: Conventional epoxy resin sheet plastination
| Autor: | Carlos Baptista, Rafael Latorre, Octavio López-Albors, Mircea-Constantin Sora, Kees de Jong |
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| Rok vydání: | 2019 |
| Předmět: |
Materials science
040301 veterinary sciences Slice thickness Anatomical structures History 21st Century Plastination 0403 veterinary science 03 medical and health sciences Anatomical concepts Animals Humans Composite material Curing (chemistry) 0303 health sciences General Veterinary Epoxy Resins 04 agricultural and veterinary sciences General Medicine Epoxy History 20th Century Sectional Anatomy 030301 anatomy & morphology visual_art visual_art.visual_art_medium Temperature sensitive Anatomy |
| Zdroj: | Anatomia, histologia, embryologiaREFERENCES. 48(6) |
| ISSN: | 1439-0264 |
| Popis: | Epoxy plastination techniques were developed to obtain thin transparent body slices with high anatomical detail. This is facilitated because the plastinated tissue is transparent and the topography of the anatomical structures well preserved. For this reason, thin epoxy slices are currently used for research purposes in both macroscopic and microscopic studies. The protocol for the conventional epoxy technique (E12) follows the main steps of plastination-specimen preparation, dehydration, impregnation and curing/casting. Preparation begins with selection of the specimen, followed by freezing and slicing. Either fresh or fixed (embalmed) tissue is suitable for epoxy plastination, while slice thickness is kept between 1.5 and 3 mm. Impregnation mixture is made of epoxy E12 resin plus E1 hardener (100 ppw; 28 ppw). This mixture is reactive and temperature sensitive, and for this reason, total impregnation time under vacuum at room laboratory temperature should not last for more than 20-24 hr. Casting of impregnated slices is done in either flat chambers or by the so-called sandwich method in either fresh mixture or the one used for impregnation. Curing is completed at 40°C to allow a complete polymerization of the epoxy-mixture. After curing, slices can be photographed, scanned or used for anatomical study under screen negatoscope, magnification glass or fluorescent microscope. Based on epoxy sheet plastination, many anatomical papers have recent observations of and/or clarification of anatomical concepts in different areas of medical expertice. |
| Databáze: | OpenAIRE |
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