Isolation ofrfbGene Clusters Directing the Synthesis of O Polysaccharides Consisting of Mannose Homopolymers and Serological Analysis of Lipopolysaccharides
| Autor: | Atsushi Saeki, Toshihide Iwashita, Tsuyoshi Sugiyama, Kei-ichi Uchiya, Nobuo Kato, Michio Ohta, Nobuo Kido |
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| Rok vydání: | 1993 |
| Předmět: |
Lipopolysaccharides
Klebsiella Antigenicity Molecular Sequence Data Immunology Mannose medicine.disease_cause Microbiology chemistry.chemical_compound Plasmid Virology Carbohydrate Conformation Escherichia coli medicine biology Strain (chemistry) Polysaccharides Bacterial O Antigens biology.organism_classification Molecular biology Enterobacteriaceae Carbohydrate Sequence chemistry Genes Bacterial Multigene Family lipids (amino acids peptides and proteins) Bacteria |
| Zdroj: | Microbiology and Immunology. 37:601-606 |
| ISSN: | 0385-5600 |
| DOI: | 10.1111/j.1348-0421.1993.tb01682.x |
| Popis: | Four serotypes of two genera, Escherichia coli O8 and O9 and Klebsiella O3 and O5, produce the O polysaccharides consisting of mannose homopolymers. Previously, we reported the isolation and expression of E. coli O9 rfb in E. coli K-12 strains (Kido et al, J. Bacteriol., 171: 3629-3633, 1989). In this study, R' plasmids carrying his-rfb region of the other three strains were isolated and expressed in E. coli K-12 strain. Serological study of lipopolysaccharides (LPS) synthesized in E. coli K-12 strain was carried out. His-linked rfb genes from E. coli O9 and Klebsiella O3 directed the synthesis of O polysaccharides with the same antigenicity as those of the parental strains in E. coli K-12 strain. On the other hand, rfb genes from E. coli O8 and Klebsiella O5 directed the synthesis of O polysaccharides which were antigenically not identical but partially common to those of the parental strains. A rough strain derived from E. coli O8 synthesized LPS which showed the identical antigenicity as the wild strain when the his-rfb region of E. coli O8 was introduced. The results suggest that some genes located distantly from his are additionally required to complete the synthesis of O polysaccharides of E. coli O8 and Klebsiella O5. |
| Databáze: | OpenAIRE |
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