Neonatal hyperoxia induces alterations in neurotrophin gene expression

Autor: Tomoko Sengoku, K.M. Murray, M.E. Wilson
Rok vydání: 2015
Předmět:
DNA (Cytosine-5-)-Methyltransferase 1
Male
medicine.medical_specialty
Nerve Tissue Proteins
Hyperoxia
Biology
Mice
03 medical and health sciences
0302 clinical medicine
Developmental Neuroscience
Pregnancy
Neurotrophic factors
030225 pediatrics
Internal medicine
Glial Fibrillary Acidic Protein
Basic Helix-Loop-Helix Transcription Factors
medicine
Glial cell line-derived neurotrophic factor
Animals
Humans
DNA (Cytosine-5-)-Methyltransferases
Glial Cell Line-Derived Neurotrophic Factor
RNA
Messenger
Cerebral Cortex
Brain-derived neurotrophic factor
Analysis of Variance
Glial fibrillary acidic protein
Brain-Derived Neurotrophic Factor
Calcium-Binding Proteins
Microfilament Proteins
Infant
Newborn
Gene Expression Regulation
Developmental
Oligodendrocyte Transcription Factor 2
Mice
Inbred C57BL
Endocrinology
medicine.anatomical_structure
nervous system
Cerebral cortex
Phosphopyruvate Hydratase
biology.protein
Female
medicine.symptom
Neuroscience
Neural development
030217 neurology & neurosurgery
Developmental Biology
Neurotrophin
Zdroj: International Journal of Developmental Neuroscience. 48:31-37
ISSN: 1873-474X
0736-5748
DOI: 10.1016/j.ijdevneu.2015.11.003
Popis: Each year in the United States, nearly 500,000 infants a year are born prematurely. Babies born before 35 weeks gestation are often placed on ventilators and/or given supplemental oxygen. This increase in oxygen, while critical for survival, can cause long-term damage to lungs, retinas and brains. In particular, hyperoxia causes apoptosis in neurons and alters glial activity. Brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) are members of the neurotrophin family of proteins that function to promote the growth, differentiation and development of the nervous system. We hypothesized that hyperoxia can alter the regulation of these genes and by doing so adversely affect the development of the brain. We predicted that mice exposed to hyperoxic conditions would have differences in BDNF and GDNF mRNA expression and relative level of methylated promoter regions coinciding with differences in the relative levels of DNMT1 and DNMT3a mRNA expression. To test this hypothesis, newborn C57Bl/6 mice and their littermates were placed in hyperoxic or normoxic conditions from postnatal day 7 to 12. There were significant decreases in BDNF mRNA expression in the prefrontal cortex following hyperoxia, but a significant increase in the isocortex. GDNF mRNA expression was significantly increased in both the isocortex and prefrontal cortex following hyperoxia. DNMT1 mRNA expression was significantly decreased in the isocortex but significantly increased in the prefrontal following hyperoxia. Together these data suggest that short-term exposure to hyperoxic conditions can affect the regulation and expression of BDNF and GDNF potentially leading to alterations in neural development.
Databáze: OpenAIRE
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