Differential expression of zinc transporters accompanies the differentiation of C2C12 myoblasts
Autor: | Juan G. Navea, Julia Quintana, Yao Xiao, Daniella Cangussu, Sonia Ortiz-Miranda, Teresita Padilla-Benavides, Cristina Tavera-Montañez, Amanda L. Paskavitz |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Blotting Western Biochemistry Article Cell Line Inorganic Chemistry Myoblasts 03 medical and health sciences Mice Myocyte Animals Homeostasis Cation Transport Proteins Myogenesis Chemistry Transporter Cell Differentiation Compartmentalization (psychology) Subcellular localization Immunohistochemistry Cell biology Cytosol Zinc 030104 developmental biology Molecular Medicine Carrier Proteins C2C12 Intracellular |
Zdroj: | Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS). 49 |
ISSN: | 1878-3252 |
Popis: | Zinc transporters facilitate metal mobilization and compartmentalization, playing a key role in cellular development. Little is known about the mechanisms and pathways of Zn movement between Zn transporters and metalloproteins during myoblast differentiation. We analyzed the differential expression of ZIP and ZnT transporters during C2C12 myoblast differentiation. Zn transporters account for a transient decrease of intracellular Zn upon myogenesis induction followed by a gradual increase of Zn in myotubes. Considering the subcellular localization and function of each of the Zn transporters, our findings indicate that a fine regulation is necessary to maintain correct metal concentrations in the cytosol and subcellular compartments to avoid toxicity, maintain homeostasis, and for loading metalloproteins needed during myogenesis. This study advances our basic understanding of the complex Zn transport network during muscle differentiation. |
Databáze: | OpenAIRE |
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