Effects of products from macrophages, blood mononuclear cells and or retinol on collagenase secretion and collagen synthesis in chondrocyte culture
| Autor: | U. Trechsel, John J. Reynolds, Gillian Murphy, G. Dew |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Cartilage
Articular Peripheral blood mononuclear cell Chondrocyte Monocytes Tissue culture medicine Animals Humans Secretion Vitamin A Molecular Biology Cells Cultured Chemistry Cartilage Macrophages Cell Biology Cell biology Kinetics medicine.anatomical_structure Microbial Collagenase Biochemistry Microbial collagenase Collagenase Collagen Rabbits Type I collagen medicine.drug |
| Zdroj: | Biochimica et biophysica acta. 720(4) |
| ISSN: | 0006-3002 |
| Popis: | Collagenase secretion was studied on cultures of rabbit articular chondrocytes. Differentiation of the cells was assessed by characterizing the type of 3H-labelled collagen produced during treatment with (1) conditioned media from rabbit peritoneal macrophages and human blood mononuclear cells, and (2) with retinol, a potent cartilage resorbing agent in tissue culture. Conditioned media stimulated collagenase secretion. Total collagen synthesis was reduced due to a decrease of synthesis of alpha 1 chains; the amount of alpha 2 chains synthesized was unchanged. This is thought to be due to a reduction in type II synthesis. Retinol did not stimulate collagenase secretion. Total collagen synthesis was reduced by retinol. alpha 2 chain synthesis, however, was significantly increased, suggesting a switch of collagen synthesis in favor of type I collagen, and therefore, dedifferentiation. These results demonstrate that dedifferentiation of chondrocytes with respect to collagen synthesis is not necessarily associated with a stimulation of collagenase secretion. |
| Databáze: | OpenAIRE |
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