Evidence of functional bile acid signaling pathways in adipocytes
Autor: | Thomas Karrasch, Jutta Schlegel, Miriam Thomalla, Andreas Schmid, Andreas Schäffler |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Adult Lipopolysaccharides Male medicine.medical_specialty medicine.drug_class Lipolysis Adipocytes White Glycocholic acid Adipose tissue Receptors Cytoplasmic and Nuclear 030209 endocrinology & metabolism Biochemistry Receptors G-Protein-Coupled Bile Acids and Salts 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Endocrinology Adipokines Internal medicine Adipocyte 3T3-L1 Cells medicine Animals Humans Receptor Fibroblast Growth Factor Type 1 Molecular Biology ATP Binding Cassette Transporter Subfamily B Member 11 Cells Cultured Adiponectin Bile acid Chemistry Deoxycholic acid Cell Differentiation G protein-coupled bile acid receptor 030104 developmental biology Gene Expression Regulation Female |
Zdroj: | Molecular and cellular endocrinology. 483 |
ISSN: | 1872-8057 |
Popis: | Background and aim Bile acids (BA) are increasingly recognized as pleiotropic and hormone-like signaling molecules with metabolic and endocrine functions. However, the role of BA in white adipocyte physiology remains somewhat obscure. It was the aim to investigate the BA receptors (FXR, TGR5) and FGFR1 (Fibroblast growth factor receptor 1) as well as Bsep (bile salt export pump) in white adipocytes and in murine and human adipose tissue (AT) and to investigate effects of different BA species in adipocyte physiology. Patients, material and methods Receptor mRNA expression was quantified by real-time PCR in mice, humans and during 3T3-L1 pre-adipocyte differentiation. Adipokines were measured by ELISA upon stimulation by several BA. Effects of BA on TNF- and LPS-induced MCP-1 secretion and lipolysis were analyzed. TNF-induced lipolysis was investigated by glycerol assay. Results The present data provide for the first time a detailed expression profile of FXR, TGR5, FGFR1, and Bsep during adipocyte differentiation and in murine and human AT. FGFR1 expression is upregulated in adipose tissue of LPS-injected animals. Several BA regulate secretion of adipokines such as adiponectin and resistin differentially. Importantly, TNF- and LPS-induced MCP-1 release from adipocytes as well as TNF-induced lipolysis can be antagonized by cholic acid (CA) and deoxycholic acid (DCA). Conclusions The present data provide evidence of functional BA signaling pathways in adipocytes and argue for certain MCP-1 related anti-inflammatory effects of BA in TNF- and LPS-induced inflammation, whereas pro-inflammatory resistin is induced by CA and glycocholic acid (GCA). Systemic bile acids might represent a hormonal network regulating white adipocyte physiology including lipolysis. |
Databáze: | OpenAIRE |
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