TLR9 and NF-κB Are Partially Involved in Activation of Human Neutrophils by Helicobacter pylori and Its Purified DNA

Autor: Carmen Maldonado-Bernal, Pedro Cortés-Reynosa, Eduardo Pérez Salazar, Javier Torres, Norma Sánchez-Zauco, Lourdes Alvarez-Arellano
Rok vydání: 2014
Předmět:
Bacterial Diseases
Neutrophils
lcsh:Medicine
Pathogenesis
Pathology and Laboratory Medicine
chemistry.chemical_compound
Medicine and Health Sciences
lcsh:Science
Receptor
Immune Response
Cells
Cultured
Innate Immune System
Multidisciplinary
NF-kappa B
Bacterial Pathogens
Infectious Diseases
medicine.anatomical_structure
Medical Microbiology
Host-Pathogen Interactions
Cytokines
medicine.symptom
Research Article
Adult
DNA
Bacterial
Immunology
Inflammation
Biology
Microbiology
Helicobacter Infections
Immune Activation
Immune system
medicine
Gastric mucosa
Humans
Gram Negative Bacteria
Microbial Pathogens
Lamina propria
Helicobacter pylori
lcsh:R
Immunity
Biology and Life Sciences
TLR9
Bacteriology
NF-κB
Molecular Development
biology.organism_classification
chemistry
Toll-Like Receptor 9
Immune System
lcsh:Q
Developmental Biology
Zdroj: PLoS ONE, Vol 9, Iss 7, p e101342 (2014)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0101342
Popis: Helicobacter pylori infection represents one of the most common bacterial infections worldwide. The inflammatory response to this bacterium involves a large influx of neutrophils to the lamina propria of the gastric mucosa. However, little is known about the receptors and molecular mechanisms involved in activation of these neutrophils. In this study, we aimed to determine the role of toll-like receptor 9 (TLR9) in the response of human neutrophils to H. pylori and purified H. pylori DNA (Hp-DNA). Neutrophils were isolated from the blood of adult volunteers and challenged with either H. pylori or Hp-DNA. We found that both, H. pylori and Hp-DNA induced increased expression and release of IL-8. Furthermore, we showed that TLR9 is involved in the induction of IL-8 production by H. pylori and Hp-DNA. IL-8 production induced by H. pylori but not by Hp-DNA was partially mediated by NF-κB. In conclusion, this study showed for first time that both, H. pylori and Hp-DNA activate TLR9 and induce a different inflammatory response that leads to activation of neutrophils.
Databáze: OpenAIRE
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