Escherichia colitRNA 2-selenouridine synthase (SelU) converts S2U-RNA to Se2U-RNAviaS-geranylated-intermediate
| Autor: | Patrycja Komar, Barbara Nawrot, Klaudia Sadowska, Ewa Radzikowska-Cieciura, Grazyna Leszczynska, Malgorzata Sierant, Elzbieta Sochacka |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Stereochemistry Thiouridine Biophysics medicine.disease_cause Biochemistry Catalysis Selenium 03 medical and health sciences Polyisoprenyl Phosphates RNA Transfer Structural Biology Organoselenium Compounds Escherichia coli Genetics medicine 2-selenouridine Uridine Molecular Biology chemistry.chemical_classification Binding Sites 030102 biochemistry & molecular biology ATP synthase biology Terpenes RNA Cell Biology biology.organism_classification Carbon RNA Bacterial 030104 developmental biology Enzyme chemistry Sulfurtransferases Transfer RNA biology.protein Protein Processing Post-Translational TRNA 2-selenouridine synthase Bacteria |
| Zdroj: | FEBS Letters. 592:2248-2258 |
| ISSN: | 0014-5793 |
| Popis: | To date the only tRNAs containing nucleosides modified with a selenium (5-carboxymethylaminomethyl-2-selenouridine and 5-methylaminomethyl-2-selenouridine) have been found in bacteria. By using tRNA anticodon-stem-loop fragments containing S2U, Se2U, or geS2U, we found that in vitro tRNA 2-selenouridine synthase (SelU) converts S2U-RNA to Se2U-RNA in a two-step process involving S2U-RNA geranylation (with ppGe) and subsequent selenation of the resulting geS2U-RNA (with SePO33- ). No 'direct' S2U-RNA→Se2U-RNA replacement is observed in the presence of SelU/SePO33- only (without ppGe). These results suggest that the in vivo S2U→Se2U and S2U→geS2U transformations in tRNA, so far claimed to be the elementary reactions occurring independently in the same domain of the SelU enzyme, should be considered a combination of two consecutive events - geranylation (S2U→geS2U) and selenation (geS2U→Se2U). |
| Databáze: | OpenAIRE |
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