Magnetic Gold Nanoparticles: Synthesis, Characterization and Its Application in the Delivery of FITC Into KG-1 Cells
| Autor: | Yuzhi Hao, Jie Chen, Steven Song, James Xing, Xiaoyan Yang |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Magnetic force
Materials science fluorescein isothiocyanate Confocal Sonication Biomedical Engineering Core shell structure Metal nanoparticles Nanoparticle Bioengineering Spherical morphologies Microscopy Atomic Force confocal microscopy fluorescence microscopy Cell Line Magnetics chemistry.chemical_compound Isothiocyanates Organic acids Humans General Materials Science Flow cytometry Fluorescein isothiocyanate Micropores Microscopy Confocal atomic force microscopy Confocal imaging General Chemistry Condensed Matter Physics Ascorbic acid Microscopy Fluorescence chemistry Chemical engineering Colloidal gold Synthesis (chemical) Magnetic nanoparticles Iron oxide nanoparticle Sonication method Gold Fluorescein-5-isothiocyanate Solution treatments Iron oxide nanoparticles |
| Zdroj: | Journal of Nanoscience and Nanotechnology. 12:7716-7722 |
| ISSN: | 1533-4880 |
| DOI: | 10.1166/jnn.2012.6647 |
| Popis: | In this article, we report a new method\-a sonication method to disperse iron oxide nanoparticles into smaller nanoparticles and make gold ions absorb onto the surface or trapped in the micropores of the iron oxide nanoparticles using sonication action. By using quick reduction of ascorbic acid and post-HCl solution treatment, gold covered magnetic nanoparticles (mGNPs) with spherical morphology and uniform size were synthesized in a water solution. The size of the mGNPs was found to be 20-30 nm. Some ideal mGNPs possessed a core-shell structure. The mGNPs were non-cytotoxic and mGNP-fluorescein isothiocyanate (FITC) can enter KG-1 cells when driven by an external magnetic force, which was confirmed by confocal imaging. The confocal image also showed the FITC inside the KG-1 cells was near the nucleus. The fluorescein isothiocyanate (FITC) delivery efficiency is about 100% according to the flow cytometry results. |
| Databáze: | OpenAIRE |
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