Increased alpha-defensin expression is associated with risk of coronary heart disease: a feasible predictive inflammatory biomarker of coronary heart disease in hyperlipidemia patients

Autor: Surachet Benjathummarak, Yaowapa Maneerat, Wilanee Dechkhajorn, Kriengchai Prasongsukarn, Urai Chaisri
Rok vydání: 2016
Předmět:
Male
0301 basic medicine
Endocrinology
Diabetes and Metabolism
Clinical Biochemistry
Gene Expression
Coronary Artery Disease
Gastroenterology
Alpha defensin
Coronary artery disease
0302 clinical medicine
Endocrinology
HNP 1–3
Hyperlipidemia
Oligonucleotide Array Sequence Analysis
Neutrophil
Middle Aged
Coronary Vessels
Coronary heart disease
030220 oncology & carcinogenesis
alpha-defensin
Biomarker (medicine)
Female
medicine.symptom
Lipidology
Adult
alpha-Defensins
medicine.medical_specialty
Alpha (ethology)
Hyperlipidemias
Inflammation
Sudden death
03 medical and health sciences
Internal medicine
medicine
Humans
RNA
Messenger
Triglycerides
Aged
Biochemistry
medical
business.industry
Gene Expression Profiling
Research
Cholesterol
HDL
Biochemistry (medical)
Cholesterol
LDL
Biomarker
Atherosclerosis
Microarray Analysis
medicine.disease
Cross-Sectional Studies
CHD
030104 developmental biology
Immunology
Leukocytes
Mononuclear
business
Biomarkers
Zdroj: Lipids in Health and Disease
ISSN: 1476-511X
DOI: 10.1186/s12944-016-0285-5
Popis: Background Atherosclerosis is a multifactorial disorder of the heart vessels that develops over decades, coupling inflammatory mechanisms and elevated total cholesterol levels under the influence of genetic and environmental factors. Without effective intervention, atherosclerosis consequently causes coronary heart disease (CHD), which leads to increased risk of sudden death. Polymorphonuclear neutrophils play a pivotal role in inflammation and atherogenesis. Human neutrophil peptides (HNPs) or alpha (α)-defensins are cysteine-rich cation polypeptides that are produced and released from activated polymorphonuclear neutrophil granules during septic inflammation and acute coronary vascular disorders. HNPs cause endothelial cell dysfunction during early atherogenesis. In this cross-sectional study, control, hyperlipidemia and CHD groups were representative as atherosclerosis development and CHD complications. We aimed to assess the correlation between α-defensin expression and the development of CHD, and whether it was a useful predictive indicator for CHD risk. Methods First, DNA microarray analysis was performed on peripheral blood mononuclear cells (PBMCs) from Thai control, hyperlipidemia and CHD male patients (n = 7). Gene expression profiling revealed eight up-regulated genes common between hyperlipidemia and CHD patients, but not controls. We sought to verify and compare α-defensin expression among the groups using: 1) real-time quantitative RT-PCR (qRT-PCR) to determine α-defensin mRNA expression (n = 10), and 2) enzyme-linked immunosorbent assay to determine plasma HNP 1–3 levels (n = 17). Statistically significant differences and correlations between groups were determined by the Mann–Whitney U test or the Kruskal–Wallis test, and the Rho-Spearman correlation, respectively. Results We found that α-defensin mRNA expression increased (mean 2-fold change) in the hyperlipidemia (p = 0.043) and CHD patients (p = 0.05) compared with the controls. CHD development moderately correlated with α-defensin mRNA expression (r = 0.429, p = 0.023) and with plasma HNP 1–3 levels (r = 0.486, p = 0.000). Conclusions Increased α-defensin expression is a potential inflammatory marker that may predict the risk of CHD development in Thai hyperlipidemia patients.
Databáze: OpenAIRE
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