Chicken Microchromosomes in the Lampbrush Phase: A Cytogenetic Description
| Autor: | Svetlana Galkina, Aleksandra Daks, Alsu F. Saifitdinova, Valerie Fillon, Elena Gaginskaya, Elena I. Koshel, Alexander Dyomin, Maria Kulak |
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| Přispěvatelé: | Biol Fac, Oranienbaumskoye Shosse 2, Saint Petersburg State University, St Petersburg Assoc Scientists & Scholars, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT], Int Ctr Reprod Med, École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Chromosomes Artificial Bacterial Chromomere gallus gallus domesticus [SDV]Life Sciences [q-bio] Zoology Biology fish mapping Chromosomes 03 medical and health sciences Cytogenetics Meiosis Lateral loop microchromosone Genetics medicine cytogenetic map Animals bac clone Molecular Biology Metaphase Genetics (clinical) Karyotype 030104 developmental biology Lampbrush chromosome Evolutionary biology Microchromosome identification lampbrush chromosome genetic Chickens |
| Zdroj: | Cytogenetic and Genome Research Cytogenetic and Genome Research, Karger, 2017, 152 (1), pp.46-54. ⟨10.1159/000475563⟩ |
| ISSN: | 1424-859X 1424-8581 |
| DOI: | 10.1159/000475563⟩ |
| Popis: | Lampbrush chromosomes are giant, transcriptionally active, meiotic chromosomes found in oocytes of all vertebrates with the exception of mammals. Lampbrush chromosomes offer a convenient tool for cytogenetic mapping and, in particular, have been instrumental in mapping genes and linkage groups on chicken (GGA) chromosomes. Whereas cytogenetic maps of macrochromosome GGA1-10 and microchromosome GGA11-16 lampbrush bivalents have been established, identification and description of smaller microchromosome bivalents are still missing. In this work, we used specific FISH probes for the identification of 12 chicken lampbrush chromosomes formed by GGA17-28. Our observations on chromomere and lateral loop arrangement and chiasma position allowed us to construct the respective cytogenetic maps for these microchromosomes. For the 10 smallest chicken microchromosomes, GGA29-38, no individual molecular tags are available, yet they can be collectively marked using the PO41 repeat. The reported results contribute to building of working cytogenetic maps of the chicken karyotype. (C) 2017 S.Karger AG, BaselKeywords |
| Databáze: | OpenAIRE |
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