Adding mucins to an in vitro batch fermentation model of the large intestine induces changes in microbial population isolated from porcine feces depending on the substrate

Autor: Georges Daube, Carine Nezer, Christelle Boudry, Jérôme Bindelle, André Thewis, Bernard Taminiau, Daniel Portetelle, Thi Hanh Tham Tran, Nadia Everaert
Rok vydání: 2015
Předmět:
Zdroj: FEMS microbiology ecology. 92(2)
ISSN: 1574-6941
Popis: Adding mucus to in vitro fermentation models of the large intestine shows that some genera, namely lactobacilli, are dependent on host–microbiota interactions and that they rely on mucosal layers to increase their activity. This study investigated whether this dependence on mucus is substrate dependent and to what extent other genera are impacted by the presence of mucus. Inulin and cellulose were fermented in vitro by a fecal inoculum from pig in the presence or not of mucin beads in order to compare fermentation patterns and bacterial communities. Mucins increased final gas production with inulin and shifted short-chain fatty acid molar ratios ( P
Databáze: OpenAIRE