NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47
| Autor: | Takeshi Sekiguchi, Toshiya Hayano, Mitsuaki Yanagida, Nobuhiro Takahashi, Takeharu Nishimoto |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
DEAD box
Nucleolus Molecular Sequence Data Ribosome biogenesis Biology Ribosome DEAD-box RNA Helicases Genetics RNA Precursors Humans Amino Acid Sequence RNA Processing Post-Transcriptional Ribosomal DNA Molecular Biology Binding Sites Base Sequence RNA Nuclear Proteins RNA Helicase A Cell biology RNA Ribosomal Carrier Proteins Ribosomes DDX47 Cell Nucleolus RNA Helicases HeLa Cells Protein Binding |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | Previously, we described a novel nucleolar protein, NOP132, which interacts with the small GTP binding protein RRAG A. To elucidate the function of NOP132 in the nucleolus, we identified proteins that interact with NOP132 using mass spectrometric methods. NOP132 associated mainly with proteins involved in ribosome biogenesis and RNA metabolism, including the DEAD-box RNA helicase protein, DDX47, whose yeast homolog is Rrp3, which has roles in pre-rRNA processing. Immunoprecipitation of FLAG-tagged DDX47 co-precipitated rRNA precursors, as well as a number of proteins that are probably involved in ribosome biogenesis, implying that DDX47 plays a role in pre-rRNA processing. Introduction of NOP132 small interfering RNAs induced a ring-like localization of DDX47 in the nucleolus, suggesting that NOP132 is required for the appropriate localization of DDX47 within the nucleolus. We propose that NOP132 functions in the recruitment of pre-rRNA processing proteins, including DDX47, to the region where rRNA is transcribed within the nucleolus. |
| Databáze: | OpenAIRE |
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