Eukaryotic DNA methylases and their use forin vitromethylation
| Autor: | H. M. I. Yesufu, A. Smart, Roger L. P. Adams, M. Bryans, A. Rinaldi |
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| Rok vydání: | 1990 |
| Předmět: |
Molecular Sequence Data
Eukaryotic DNA replication Biology Methylation Chromatography Affinity Substrate Specificity Mice chemistry.chemical_compound Animals DNA Modification Methylases chemistry.chemical_classification Plants Medicinal High molecular mass Base Sequence Fabaceae General Medicine In vitro Molecular Weight Kinetics Enzyme Liver chemistry CpG site Biochemistry Chromatography Gel DNA |
| Zdroj: | Philosophical Transactions of the Royal Society of London. B, Biological Sciences. 326:189-198 |
| ISSN: | 2054-0280 0080-4622 |
| DOI: | 10.1098/rstb.1990.0003 |
| Popis: | DNA methylases from mouse and pea have been purified and characterized. Both are high molecular mass enzymes that show greater activity with hemimethylated than unmethylated substrate DNA. Both methylate cytosines in CpG preferentially, but not exclusively and show similar kinetics of methylation, which makes it difficult to saturate all possible sites on the DNA, but procedures are described that circumvent this problem. |
| Databáze: | OpenAIRE |
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