Partial purification and characterization of a thermostable β-mannanase from Aspergillus foetidus
| Autor: | Edivaldo Ximenes Ferreira Filho, Carlos Frederico de Souza Castro, Maria de Lourdes Teixeira de Moraes Polizeli, Juliana da Conceição Infante de Marco, Geraldo Pereira de Souza Neto, Michele Michelin |
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| Rok vydání: | 2015 |
| Předmět: |
Sodium
Size-exclusion chromatography Ultrafiltration chemistry.chemical_element Ethylenediaminetetraacetic acid lcsh:Technology soybean husk lcsh:Chemistry chemistry.chemical_compound β-mannanase ASPERGILLUS General Materials Science Instrumentation lcsh:QH301-705.5 Fluid Flow and Transfer Processes Aspergillus foetidus Chromatography biology lcsh:T Process Chemistry and Technology General Engineering Substrate (chemistry) biology.organism_classification lcsh:QC1-999 Computer Science Applications chemistry lcsh:Biology (General) lcsh:QD1-999 lcsh:TA1-2040 Locust bean gum lcsh:Engineering (General). Civil engineering (General) lcsh:Physics Mesophile |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Applied Sciences, Vol 5, Iss 4, Pp 881-893 (2015) Applied Sciences Volume 5 Issue 4 Pages 881-893 |
| Popis: | An extracellular β-mannanase was isolated from samples of crude extract of the mesophilic fungus Aspergillus foetidus grown on soybean husk as a carbon source. The induction profile showed that β-mannanase reached a maximum activity level (2.0 IU/mL) on the 15th day of cultivation. The enzyme was partially purified by ultrafiltration and gel filtration chromatography procedures and was named Man 58. Sodium dodecyl sulfate-polyacrilamide electrophoresis and zymogram analysis of Man 58 showed two bands of approximately 43 and 45 kDa with β-mannanase activity. Ultrafiltration showed that β-mannanase activity was only detected in the concentrated sample. Man 58 was most active at 60 °C and at pH 4.0. It was thermostable in the temperature range of 40–60 °C for eleven days, and the half-life at 70 °C was ten days. Man 58 showed Km and Vmax values of 3.29 mg/mL and 1.76 IU/mL respectively, with locust bean gum as a substrate. It was mostly activated by FeSO4 and CoCl2 and inhibited by MgSO4, FeCl3, CuSO4, MgCl2, ZnCl2, ZnSO4, CaCl2, CuCl2, KCl and ethylenediaminetetraacetic acid (EDTA). Phenolic compounds did not inhibit the enzyme. On the other hand, auto-hydrolysis liquor showed an inhibitory effect on Man 58 activity. |
| Databáze: | OpenAIRE |
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